20.500.12556/RUL-128315 Two-photon retinal theranostics by adaptive compact laser source To avoid a devastating effect of eye vision impairment on the information flow from the eye to our brain, enormous effort is being put during the last decades into the development of more sensitive diagnostics and more efficient therapies of retinal tissue. While morphology can be impressively imaged by optical coherence tomography, molecular-associated pathology information can be provided almost exclusively by auto-fluorescence-based methods. Among the latter, the recently developed fluorescence lifetime imaging ophthalmoscopy (FLIO) has the potential to provide both structural information and interacting pictures at the same time. The requirements for FLIO laser sources are almost orthogonal to the laser sources used in phototherapy that is expected to follow up the FLIO diagnostics. To make theranostics more effective and cheaper, the complete system would need to couple at least the modalities of low-power high-repetition-rate FLIO and precision high-pulse energy-adjustable repetition rate phototherapy. In addition, the intermediate-power high repetition rate for two-photon excitation would also be desired to increase the depth resolution. In our work, compact fiber-laser based on high-speed gain-switched laser diode has been shown to achieve adaptable/independently tunable repetition rate and energy per pulse allowing coupled fluorescence lifetime diagnostics via two-photon excitation and phototherapy via laser-induced photodisruption on a local molecular environment in a complex ex vivo retinal tissue. adaptable fiber lasers retinal tissue theranostics multimodal imaging fluorescence lifetime imaging prilagodljivi vlakenski laserji tkivo mrežnice teranostika multimodalno slikanje življenjski čas fluorescence true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-08 10:39:47 2021-07-08 10:39:53 2022-09-07 04:14:23 0000-00-00 00:00:00 2020 0 0 Str. 1-9 iss. 6 Vol. 126, art. 405 2020 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2021-05-11 535(045) 0947-8396 10.1007/s00339-020-03587-2 14993923 RAZ_Podlipec_Rok_2020.pdf RAZ_Podlipec_Rok_2020.pdf 1 E0CEC0117141A105DFB0FD015AB23576 3ce52007708b7f23a8e032fdd0c0171aa4bf4e2f67ca6672c0daf2478ffd5eed 5b3366b2-dfc8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145015 https://link.springer.com/article/10.1007/s00339-020-03587-2 1 ef0fe8ab-dfc7-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145014 Fakulteta za strojništvo 0 0 0