20.500.12556/RUL-128311 Accumulation of agmatine, spermidine, and spermine in sprouts and microgreens of alfalfa, fenugreek, lentil, and daikon radish Sprouts and microgreens are a rich source of various bioactive compounds. Seeds of lentil, fenugreek, alfalfa, and daikon radish seeds were germinated and the contents of the polyamines agmatine (AGM), putrescine (PUT), cadaverine (CAD), spermidine (SPD), and spermine (SPM) in ungerminated seeds, sprouts, and microgreens were determined. In general, sprouting led to the accumulation of the total polyamine content. The highest levels of AGM (5392 mg/kg) were found in alfalfa microgreens, PUT (1079 mg/kg) and CAD (3563 mg/kg) in fenugreek sprouts, SPD (579 mg/kg) in lentil microgreens, and SPM (922 mg/kg) in fenugreek microgreens. A large increase in CAD content was observed in all three legume sprouts. Conversely, the nutritionally beneficial polyamines AGM, SPD, and SPM were accumulated in microgreens, while their contents of CAD were significantly lower. In contrast, daikon radish sprouts exhibited a nutritionally better profile of polyamines than the microgreens. Freezing and thawing of legume sprouts resulted in significant degradation of CAD, PUT, and AGM by endogenous diamine oxidases. The enzymatic potential of fenugreek sprouts can be used to degrade exogenous PUT, CAD, and tyramine at pH values above 5. polyamines biogenic amines germination medicago sativa trigonella foenum-graecum lens culinaris raphanus sativus diamine oxidase biogeni amini poliamini agmatin spermidin spermin kalčki kalitev ekstrakcija sušenje zmrzovanje tajanje kromatografske metode HPLC true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-08 09:37:20 2021-07-08 09:37:21 2022-09-07 04:14:22 0000-00-00 00:00:00 2020 0 0 20 str. iss. 5, art. 547 Vol. 9 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.1:547.233:641.1:543.544 2304-8158 10.3390/foods9050547 13634563 RAZ_Kralj_Cigic_Irena_2020.pdf RAZ_Kralj_Cigic_Irena_2020.pdf 1 85D023EA3FFB54A2B4D39181007E1065 ce2c73a06f98ab3b5540c3c33ef36353e2cbd7d136f1988ad963b5dd924475ca 66208583-dfbf-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145007 https://www.mdpi.com/2304-8158/9/5/547 1 31f51b53-dfbf-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145006 Fakulteta za kemijo in kemijsko tehnologijo Biotehniška fakulteta 0 0 0