20.500.12556/RUL-128261 In vitro comparison of the bioactivities of Japanese and Bohemian knotweed ethanol extracts Knotweed is a flowering plant that is native to temperate and subtropical regions in the northern hemisphere. We evaluated Japanese (Reynoutria japonica Houtt.) and Bohemian (Fallopia x bohemica) knotweed rhizome and flower ethanol extracts and compared them in terms of their biological activities. The specific polyphenols were identified and quantified using HPLC/DAD, and the antioxidant activity was determined using 2,2-diphenly-1-picrylhydrazyl (DPPH) and cellular antioxidant capacity assays. The anticancer activity was evaluated as the difference between the cytotoxicity to cancer cells compared with control cells. The antimicrobial activity was determined using bacteria and yeast. The antidiabetic activity was tested as the ability of the extracts to inhibit α-amylase. Both rhizome extracts were sources of polyphenols, particularly polydatin and (−)-epicatechin; however, the cellular assay showed the highest antioxidant capacity in the flower extract of F. bohemica. The PaTu cell line was the least sensitive toward all knotweed extracts. The flower extracts of both species were less toxic than the rhizomes. However, the activity of the tested extracts was not specific for cancer cells, indicating a rather toxic mode of action. Furthermore, all used extracts decreased the α-amylase activity, and the rhizome extracts were more effective than the flower extracts. None of the extracts inhibited bacterial growth; however, they inhibited yeast growth. The results confirmed that rhizomes of Reynoutria japonica Houtt. could become a new source of bioactive compounds, which could be used for the co-treatment of diabetes and as antifungal agents. antioxidant activity cellular antioxidant activity polyphenols anticancer activity antimicrobial activity antidiabetic activity herbal medicine antioksidativna učinkovitost rastlinski izvlečki dresnik Fallopia bioaktivne snovi fenolne spojine polifenoli protimikrobno delovanje protirakavo delovanje antidiabetično delovanje true false true Angleški jezik Slovenski jezik Članek v reviji 2021-07-07 15:25:45 2021-07-07 15:25:46 2022-09-07 04:14:03 0000-00-00 00:00:00 2020 0 0 12 str. iss. 5, art. 544 Vol. 9 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577.1:547.9:582.665:547.56:579.24:66.094.3.097.8 2304-8158 10.3390/foods9050544 13360899 RAZ_Pogacnik_Lea_2020.pdf RAZ_Pogacnik_Lea_2020.pdf 1 C1A96AA454D4184724EF77285FD7FF42 7fa28b5d610c17df6e32417651ffa715cf2072f181121f16d560522c0af263df fd12b84b-df26-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144944 https://www.mdpi.com/2304-8158/9/5/544 1 b491d944-df26-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144943 Biotehniška fakulteta 0 0 0