Vpliv malega proteina DdrR na lastnosti LexA proteinu podobnih molekul pri bakteriji Acinetobacter baumannii
Encimi, sintetizirani v odzivu SOS, omogočijo bakterijam popravilo poškodb DNA in s tem adaptacijo na stresne okoljske razmere. Sevi bakterije Acinetobacter baumannii, ki so odporni proti številnim antibiotikom, sintetizirajo mali protein DdrR, ki je specifičen le za rod Acinetobacter, in skupaj s proteinom UmuDAb v tej bakteriji uravnava prepis genov odziva SOS. V delu smo želeli preveriti ali se protein DdrR neposredno veže s proteinom UmuDAb. Poleg tega smo želeli preveriti ali protein DdrR interagira tudi z represorjema, ki nadzirata lizogeni cikel dveh temperantnih profagov v tej bakteriji. V študiji smo z uporabo površinske plazmonske resonance ugotovili, da se DdrR z visoko afiniteto veže na vse analizirane represorje in, najverjetneje na tak način koregulira prepis bakterijskih in bakteriofagnih genov.
Enzymes, synthesized in bacterial SOS response, enable bacterial organisms to repair DNA damage and therefore easily adapt to stresful enviroments and chemical agenses. Acinetobacter baumannii strains are resistant to a large range and variety of antibiotics. During SOS response Acinetobacter baumannii synthesize small protein molecule, named DdrR, which is specific only for genus Acinetobacter and can be found no where else. When synthesized, DdrR co-regulates transcription of SOS damage inducible genes together with main bacterial protein regulator, UmuDAb. In the study we examined if DdrR binds directly to UmuDAb protein and to two other phage repressors, which regulate lizogenic cycle of two temperate prophages, situated in bacterial chromosomal DNA. Surface plasmon resonance analysis indicated that DdrR protein binded succesfully to all analyzed protein repressors, with high affinity, and, in this way, it most likely co-regulates transcription of bacterial and bacteriophage genes.
2021
2021-07-04 07:15:45
1060
bakterijski odziv SOS, Acinetobacter baumannii, regulacija genov, represor UmuDAb, mali protein DdrR, fagni represorji
bacterial SOS response, Acinetobacter baumannii, regulation of genes, UmuDAb repressor, DdrR small protein, prophage repressors
mb22
[N. Šeme]
Nuša
Šeme
70
Matej
Butala
991
UDK
4
579.25/.26:577.112:577.2.088
VisID
16
191363
COBISS_ID
3
69310467
15219.pdf
1998058
Predstavitvena datoteka
2021-07-04 07:15:49