20.500.12556/RUL-127767 The risk assessment of pesticide ingestion with fruit and vegetables for consumer's health Pesticides are chemicals used in agriculture to protect crops from pests. In addition to protection during cultivation, they are also used after harvesting to extend the shelf life of products. Postharvest control stands out, especially when it comes to products imported from distant countries, resulting in increased concentration of pesticides and risk to human health consuming such products. In this study, analyses of pesticide residues were performed on 200 samples of fruits and vegetables. Pesticide residues were identified and quantified in 30 out of 200 samples. Study results revealed imazalil to be the most frequently detected pesticide. Risk assessment was performed on the obtained results, and it was carried out separately for adults and for children under 6 years of age. Imazalil showed the highest ARfD percentage for adults (max% ARfD 251%), and these values were especially high on risk assessment for children, where they amounted up to max% ARfD 1087%. The study of imazalil impact was performed on 16 Swiss albino mice divided into two groups and 4 subgroups. Experimental group animals were treated with the corresponding NOAEL dose of imazalil (10 mg/kg) for 28 days. Body weight was measured before each pesticide application on a digital electronic Sartorius scale. Peripheral blood analysis was performed after 28-day animal exposure to pesticides. Animals were anesthetized, blood samples were obtained by cardiac puncture, and red blood cell (RBC) count, hemoglobin (Hb) concentration, and white blood cell (WBC) count were determined by standard hematological methods. The organs for determination of imazalil concentration were extracted immediately upon animal sacrifice and stored in a freezer at -80°C until analysis. Results show difference in gain weight, and an increase in WBC count was recorded in the experimental group as compared with a control group of animals. The highest imazalil levels were recorded in adipose tissue (45.2‰) which proves tendency to accumulate. human health pesticides imazalil risk assessment zdravje ljudi pesticidi imazalil ocena tveganja true false true Angleški jezik Angleški jezik Članek v reviji 2021-06-22 10:12:19 2021-06-22 10:12:19 2022-09-06 03:53:01 0000-00-00 00:00:00 2021 0 0 Str. 1-8 Vol. 2021 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 614:632.95.024 2314-5765 10.1155/2021/9990219 67802883 https://www.hindawi.com/journals/ijfs/2021/9990219/ 1 762decf9-d331-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144281 RAZ_Jurak_Gordana_2021.pdf RAZ_Jurak_Gordana_2021.pdf 1 A71F4032A420E888C4E073E53710B5EE 9750a60f705cbb70f7c515361b50019c583602b06b0b229503d919b32846d245 d229883f-d331-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=144282 Zdravstvena fakulteta 0 0 0