20.500.12556/RUL-127243 Bacterial adhesion capacity of uropathogenic Escherichia coli to polyelectrolyte multilayer coated urinary catheter surface The application of catheters to the urinary tract is associated with nosocomial infections. Such infections are one of the most common types of infections in hospitals and health care facilities and can lead to numerous medical complications. Therefore, the understanding of the properties of urinary catheter surfaces and their potential modifications are crucial in order to reduce bacterial adhesion and subsequent biofilm formation. In our study, we consider standard polyvinyl chloride (PVC) catheter surfaces and compare their properties with the properties of the same surfaces coated with poly(diallyldimethylammonium chloride)/poly(sodium 4-styrenesulfonate) (PDADMA/PSS) polyelectrolyte multilayers. Uncoated and coated surfaces were characterized by means of roughness, hydrophobicity, and zeta potential measurements. Finally, bacterial adhesion extent of uropathogenic Escherichia coli on bare and polyelectrolyte multilayer coated surfaces was measured. The obtained results show that on non-treated surfaces, biofilm is formed which was not the case for multilayer coated surfaces. The PSS-terminated multilayer shows the lowest bacterial adhesion and could be helpful in prevention of biofilm formation. The analysis of the properties of the uncoated and coated surfaces reveals that the most significant difference is related to the charge (i.e., zeta potential) of the examined surfaces, while roughness and hydrophobicity of the examined surfaces are similar. Therefore, it could be concluded that the surface charge plays the crucial role in the bacterial adhesion on uncoated and coated PVC catheter surfaces. urinary catheters polyelectrolyte multilayers bacterial adhesion Escherichia coli true false true Angleški jezik Ni določen Članek v reviji 2021-05-28 11:57:50 2021-05-28 11:57:51 2023-06-09 13:38:30 0000-00-00 00:00:00 2021 0 0 12 str. iss. 6, art. 630 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 544.722.54:615.472.5 2079-6412 10.3390/coatings11060630 64997379 RAZ_Bohinc_Klemen_2021.pdf RAZ_Bohinc_Klemen_2021.pdf 1 9C32075AC16110001C649D70AAB243D5 8fd3c64d369fdb09ab3367418c33ea6f65f8df55d6e8d628b7f0c87a5e457b67 f96ddb7e-ac28-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154915 https://www.mdpi.com/2079-6412/11/6/630 1 19fe9cbb-bf9b-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=143546 Zdravstvena fakulteta 0 0 0