20.500.12556/RUL-127091 A rapid analysis of bisphenol A using MISPE coupled with HPLC-FLD in tissues of food-producing animals Bisphenol A (BPA) is a highly-produced organic compound of anthropogenic origin with a wide-range use and is ubiquitously present in both living organisms and the environment. A previously published analytical method for testing of the free (aglycone) BPA in foodstuffs was simplified and optimized for sheep muscle tissue, kidney and liver, by using only a single MISPE purification step allied with HPLC-FLD analysis. The recovery rates and RSD values over a concentration range of 1–10 μg/kg were in the range of 67–86% and 3–34%, respectively, while linearity in the matrix, represented by the r$^2$, was ≥0.999. LOD values were 0.5–1 μg/kg and LOQ values were 1 μg/kg. The analytical method used is a contribution to the field of veterinary toxicology and food testing and also proved to be applicable for other food-producing animal species, e.g. pigs, poultry and freshwater fish. The MISPE sorbent material for testing of BPA was reusable, with up to five re-use cycles without significant loss in performance characteristics. •The paper reports on a rapid determination of free (aglycone) BPA in tissues of food-producing animals by HPLC-FLD and presents a substantial improvement of analytical performance with regard to time- and cost-savings, as well as environmental protection. •MISPE is an advanced analytical technology, and the results proved that in a single and simplified SPE step it had good performance characteristics such as selectivity, recovery and precision, allied with low LOD and LOQ values. •It was proved that MISPE BPA cartridges could be used at least five times without significant loss of methodological recovery and precision. bisphenol A tissue analysis solid phase extraction molecularly imprinted polymers liquid chromatography bisphenol A-glycidyl methacrylate true false true Angleški jezik Ni določen Članek v reviji 2021-05-17 10:45:15 2021-05-17 10:45:15 2022-09-06 03:49:43 0000-00-00 00:00:00 2021 0 0 14 str. art. 101351 Vol. 8 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.9 2215-0161 10.1016/j.mex.2021.101351 62349827 RAZ_Cerkvenik_Vesna_2021.pdf RAZ_Cerkvenik_Vesna_2021.pdf 1 7935D569F836AE669D10837D584BFEFF a75a417c8f2de057fd8355648e661e98eb28a3a80816017d89eda7e016662e7c 55689acf-b6ed-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=143306 https://www.sciencedirect.com/science/article/pii/S2215016121001448 0 30d42582-196e-11ed-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=159175 Veterinarska fakulteta 0 0 0