20.500.12556/RUL-126677 The limitations of hydrodynamic removal of biofilms from the dead-ends in a model drinking water distribution system Biofilm formation and removal from dead-ends is a particularly difficult and understudied area of water distribution system biology. In this work, we have built a model drinking water distribution system to probe the effect of different hydrodynamic flow regimes on biofilm formation and removal in the main pipe and in the dead-end. The test rig was built to include all major drinking water distribution system components with materials and dimensions used in standard plumbing systems. We have simulated the effect of stagnant, laminar, turbulent, and intense turbulent flushing conditions on the growth and removal of biofilms from the main pipe and the dead-end. The growth of the biofilm in the main pipe was not prevented at a volumetric flow rate of 9.4 L min−1 and flow velocity of 2 m s−1. Mature biofilms were more difficult to remove. Biofilms grown under shear stress conditions could withstand significantly higher shear stresses than those to which they were exposed to during growth. The biofilms grew twice as fast in the dead-end when flow in the main pipe was turbulent compared to stagnant conditions. Biofilms in the dead-end were not affected by the flushing conditions in the main pipe (Q = 52 L min−1, Re = 9.0 · 104). The computational fluid dynamics simulation suggests that biofilms cannot be hydrodynamically removed from the dead-end at depths that are larger than one pipe diameter. Biofilms beyond this limit present a possible source for reinoculation and recolonization of the rest of the water distribution system. biofilm control drinking water distribution systems hydrodynamic flow dead-ends escherichia coli nadzor biofilmov modelni vodovodni sistemi tokovne razmere hidrodinamični tok mrtvi rokavi E. coli true false true Angleški jezik Slovenski jezik Članek v reviji 2021-05-03 13:48:06 2021-05-03 13:48:06 2025-03-14 12:42:59 0000-00-00 00:00:00 2020 0 0 Str. 1-13 Vol. 178 2020 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 2020-07-01 2022-07-01 579.842.1/.2:621.643 0043-1354 10.1016/j.watres.2020.115838 12956163 RAZ_Simunic_Urh_2020.pdf RAZ_Simunic_Urh_2020.pdf 1 E6C9FF466701D0AE63FA5A4204166CE4 3dbd237a30444e64f23d5f8cc9e62389d63a934faf12b4b84769c39f38b13021 0817e4d8-ac09-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142732 https://www.sciencedirect.com/science/article/pii/S0043135420303754?via%3Dihub 1 66fc7749-ac05-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142729 Fakulteta za strojništvo 0 0 0