20.500.12556/RUL-126636 Optimization of accelerated solvent extraction (ASE) of silver fir wood (Abies alba Mill.) Optimizacija pospešene ekstrakcije s topili (ASE) na primeru lesa jelke (Abies alba Mill.) Qualitative and quantitative analysis of wood plant extractives depend on the efficiency of the extraction method used. The aim of this study was the application of the accelerated solvent extraction system ASE 350 to obtain hydrophilic extracts form silver fir wood (Abies alba Mill.) and optimization of the extraction protocol. Silver fir trees originated from Kočevska Reka. Dissected and milled samples of sapwood, heartwood and knots were extracted with the ASE 350 with water, ethanol (95 % aq), acetone (95 % aq) and ethyl acetate. The number of extraction cycles was determined. Analysis of extracts was done by UV/Vis spectrophotometry and HPLC. The most effective solvent for the extraction of phenolic components from heartwood was ethanol (95 % aq), in the case of sapwood water and acetone (95 % aq) in the case of knotwood. Only two 5-minute extraction cycles were required for sufficient extraction yield when using ASE 350 at 100 °C and 103.42 bar. Kvalitativna in kvantitativna analiza ekstraktivov iz lesa je v veliki meri odvisna od učinkovitosti uporabljene ekstrakcijske metode. Cilj te študije je bil uporabiti sistem za pospešeno ekstrakcijo ASE 350 za pridobivanje hidrofilnih ekstraktivov iz lesa bele jelke (Abies alba Mill.) ter optimizirati protokol ekstrakcije. Drevesa bele jelke so bila posekana v gozdovih Kočevske Reke. Izolirane in zmlete vzorce beljave, jedrovine in grč smo ekstrahirali z ASE 350 z vodo, etanolom (95 % aq), acetonom (95 % aq) in etil acetatom. Določili smo število ekstrakcijskih ciklov. Semi-kvantitativno analizo ekstraktov smo izvedli z UV/Vis spektrofotometrijo, kvalitativno analizo pa s HPLC. Najučinkovitejše topilo za ekstrakcijo fenolnih komponent iz jedrovine je bil etanol (95 % aq), v primeru beljave je bila to voda, pri grčah pa aceton (95 % aq). Za učinkovito ekstrakcijo z uporabo ASE 350 pri 100 °C in 103,42 bar sta bila potrebna le dva 5-minutna ekstrakcijska cikla. accelerated solvent extraction wood silver fir hydrophilic extractives chromatography pospešena ekstrakcija s topili les bela jelka Abies alba hidrofilni ekstraktivi UV/Vis kromatografija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-04-29 12:25:22 2021-04-29 12:25:23 2024-04-03 07:37:15 0000-00-00 00:00:00 2019 0 0 Str. 69-77 št. 2 Letn. 68 dec. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*813.2 0024-1067 10.26614/les-wood.2019.v68n02a06 3168649 RAZ_Kerzic_Eli_2019.pdf RAZ_Kerzic_Eli_2019.pdf 1 2D0631949CE5BCD2FE88C5AF2F2A58C3 09f5c7781788630b7a1978a9d83c7b0b1163883ddc46559a0ac62b56b7a90253 8ac5dbca-a8d5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142650 https://journals.uni-lj.si/les-wood/article/view/12154 0 30d51c4b-a8d5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142649 Biotehniška fakulteta Les 0 0 0