20.500.12556/RUL-126488 Bioprospecting of less-polar constituents from endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea and targeted antioxidant effects in vitro and in vivo (zebrafish model) The endemic brown macroalga Fucus virsoides J. Agardh from the Adriatic Sea was in the focus of the present research. The volatiles of fresh (FrFv) and air-dried (DrFv) samples of F. virsoides obtained by headspace solid-phase microextraction (HS-SPME) and hydrodistillation (HD) were analyzed by gas chromatography equipped with flame ionization detector and mass spectrometry (GC-FID/MS). The major HS-FrFv compound was pentadecane (61.90–71.55%) followed by pentadec1-ene (11.00–7.98%). In HS-DrFv, pentadec-1-ene was not present, and few lower aliphatic compounds appeared, as well as benzaldehyde and benzyl alcohol. In HD-FrFv, particularly abundant were alkenes (such as pentadec-1-ene (19.32%), or (E)-pentadec-7-ene (8.35%)). In HD-DrFv, more oxidation products were present (e.g., carbonyl compounds such as tridecanal (18.51%)). The fatty acids profile of freeze-dried sample (FdFv) after conversion to methyl esters was determined by GC-FID, and oleic acid was dominant (42.28%), followed by arachidonic acid (15.00%). High-performance liquid chromatography-high-resolution mass spectrometry with electrospray ionization (HPLC-ESI-HRMS) was used for the screening of less polar fractions (F3 and F4) of F. virsoides. Mono- and diglycerides of stearic, palmitic, oleic, and arachidonic acids were found. Terpenoids and steroids comprised the compounds C$_{20}$H$_{30(32)}$O$_2$ and C$_{29}$H$_{48}$O$_{(2)}$. Among carotenoids, fucoxanthin was identified. Chlorophyll derivatives were also found (C$_{55}$H$_{74(72)}$N$_4$O$_{(5-7)}$), dominated by pheophytin a. The antioxidant activity of the fractions was investigated by in vitro assays (oxygen radical absorbance capacity (ORAC), reduction of radical cation (ABTS•$^+$), 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay, and ferric reducing antioxidant power (FRAP)) and by in vivo zebrafish model (along with fish embryotoxicity). In vitro experiments proved good radical scavenging abilities of F3 and F4 fractions, which were additionally supported by the protective effect against hydrogen peroxideinduced oxidative stress in zebrafish embryos. brown algae volatiles fatty acids pigments bioassays embryotoxicity reactive oxygen species true false true Angleški jezik Ni določen Članek v reviji 2021-04-23 13:04:33 2021-04-23 13:04:36 2022-09-06 03:48:06 0000-00-00 00:00:00 2021 0 0 25 str. iss. 5, art. 235 Vol. 19 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.51:582.272 1660-3397 10.3390/md19050235 60999427 RAZ_Jerkovic_Igor_2021.pdf RAZ_Jerkovic_Igor_2021.pdf 1 DFED744FD06BDD3E4F9A5C05AF12BF9F 8c6de5139ab0d43728a157cd20f2e784cb15c71c57c3268a4b9cdecd4eba9e64 a4ab4e24-a8e7-11ec-8aca-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=154718 https://www.mdpi.com/1660-3397/19/5/235 1 af82a55b-a423-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=142468 Zdravstvena fakulteta 0 0 0