20.500.12556/RUL-125680 Peptide signaling without feedback in signal production operates as a true quorum sensing communication system in Bacillus subtilis Bacterial quorum sensing (QS) is based on signal molecules (SM), which increase in concentration with cell density. At critical SM concentration, a variety of adaptive genes sharply change their expression from basic level to maximum level. In general, this sharp transition, a hallmark of true QS, requires an SM dependent positive feedback loop, where SM enhances its own production. Some communication systems, like the peptide SM-based ComQXPA communication system of Bacillus subtilis, do not have this feedback loop and we do not understand how and if the sharp transition in gene expression is achieved. Based on experiments and mathematical modeling, we observed that the SM peptide ComX encodes the information about cell density, specific cell growth rate, and even oxygen concentration, which ensure power-law increase in SM production. This enables together with the cooperative response to SM (ComX) a sharp transition in gene expression level and this without the SM dependent feedback loop. Due to its ultra-sensitive nature, the ComQXPA can operate at SM concentrations that are 100–1000 times lower than typically found in other QS systems, thereby substantially reducing the total metabolic cost of otherwise expensive ComX peptide. bacterial physiology microbial ecology Bacillus subtilis komunikacija med celicami zaznavanje kvoruma signalni peptidi true false true Angleški jezik Slovenski jezik Članek v reviji 2021-04-01 15:07:54 2021-04-01 15:07:54 2023-03-28 03:26:45 0000-00-00 00:00:00 2021 0 0 12 str. Vol. 4, art. 58 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.22/.26:579.852.1 2399-3642 10.1038/s42003-020-01553-5 47412483 RAZ_Dogsa_Iztok_2021.pdf RAZ_Dogsa_Iztok_2021.pdf 1 67FB03CAAD5B0C04D910AA68D69EAC87 34194d6cd4be293a19fd52b1f3ed676711a193b40b53fe99a775f9c595631a51 c442fb1d-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141515 https://www.nature.com/articles/s42003-020-01553-5 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141517 Biotehniška fakulteta 0 0 0