20.500.12556/RUL-125369 Does the urothelium of old mice regenerate after chitosan injury as quickly as the urothelium of young mice? The aging of organisms leads to a decreased ability of tissue to regenerate after injury. The regeneration of the bladder urothelium after induced desquamation with biopolymer chitosan has been studied in young mice but not in old mice. Chitosan is a suitable inducer of urothelial desquamation because it is known to be non-toxic. We used chitosan for desquamation of urothelial cells in order to compare the dynamics of urothelial regeneration after injury between young and old mice. Our aim was to determine whether the urothelial function and structure of old mice is restored as fast as in young mice, and to evaluate the inflammatory response due to chitosan treatment. We discovered that the urothelial function restored comparably fast in both age groups and that the urothelium of young and old mice recovered within 5 days after injury, although the onset of proliferation and differentiation appeared later in old mice. Acute inflammation markers showed some differences in the inflammatory response in young versus old mice, but in both age groups, chitosan caused short-term acute inflammation. In conclusion, the restoration of urothelial function is not impaired in old mice, but the regeneration of the urothelial structure in old mice slightly lags behind the regeneration in young mice. urothelium young mice old mice chitosan regeneration inflammation urotelijskih celice hitosan vnetni odgovor mlade miši regeneracija urotelija true false true Angleški jezik Slovenski jezik Članek v reviji 2021-03-12 12:08:09 2021-03-12 12:08:11 2022-09-05 03:50:26 0000-00-00 00:00:00 2020 0 0 23 str. iss. 10, art. 3502 Vol. 21 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 542:616.62 1422-0067 10.3390/ijms21103502 16463875 2779162 RAZ_Erzar_Eva_2020.pdf RAZ_Erzar_Eva_2020.pdf 1 41B5067DEBA6C77C657A67EB4203C827 45851b8428a8b8a907f5d87b4f442673c77b117ac5aecb8f604726fd8116089f b291b2dd-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141066 https://www.mdpi.com/1422-0067/21/10/3502 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141065 Fakulteta za farmacijo Medicinska fakulteta 0 0 0