20.500.12556/RUL-125362 Effects of electrospinning on the viability of ten species of lactic acid bacteria in poly(ethylene oxide) nanofibers Lactic acid bacteria can have beneficial health effects and be used for the treatment of various diseases. However, there remains the challenge of encapsulating probiotics into delivery systems with a high viability and encapsulation efficacy. The electrospinning of bacteria is a novel and little-studied method, and further investigation of its promising potential is needed. Here, the morphology, zeta potential, hydrophobicity, average cell mass, and growth characteristics of nine different species of Lactobacillus and one of Lactococcus are characterized. The electrospinning of polymer solutions containing ~10 log colony forming units (CFU)/mL lactic acid bacteria enabled the successful incorporation of all bacterial species tested, from the smallest (0.74 µm; Lactococcus lactis) to the largest (10.82 µm; Lactobacillus delbrueckii ssp. bulgaricus), into poly(ethylene oxide) nanofibers with an average diameter of ~100 nm. All of these lactobacilli were viable after incorporation into nanofibers, with 0 to 3 log CFU/mg loss in viability, depending on the species. Viability correlated with the hydrophobicity and extreme length of lactic acid bacteria, whereas a horizonal or vertical electrospinning set-up did not have any role. Therefore, electrospinning represents a promising method for the incorporation of lactic acid bacteria into solid delivery systems, while drying the bacterial dispersion at the same time. nanotechnology biotechnology probiotics Lactobacillus Lactococcus electrospinning nanofibers drying local delivery viability nanomateriali nanotehnologija biotehnologija probiotiki sušenje dostavni sistemi true false true Angleški jezik Slovenski jezik Članek v reviji 2021-03-12 11:06:49 2021-03-12 11:06:52 2022-09-05 03:50:22 0000-00-00 00:00:00 2019 0 0 14 str. iss. 9, art. 483 Vol. 11 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 620.3+60 1999-4923 10.3390/pharmaceutics11090483 4790897 517949977 RAZ_Zupancic_Spela_2019.pdf RAZ_Zupancic_Spela_2019.pdf 1 3DD16E5740355BECBE21770F4276ABB0 37eb6bd252826300827c56cd9318a01ada29a0d2af4d57deef4faaf3503ce2c0 b23dc821-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141056 https://www.mdpi.com/1999-4923/11/9/483 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141054 Fakulteta za farmacijo 0 0 0