20.500.12556/RUL-125361 Effect of solution composition variables on electrospun alginate nanofibers response surface analysis Alginate is a promising biocompatible and biodegradable polymer for production of nanofibers for drug delivery and tissue engineering. However, alginate is difficult to electrospin due to its polyelectrolyte nature. The aim was to improve the 'electrospinability' of alginate with addition of exceptionally high molecular weight poly(ethylene oxide) (PEO) as a co-polymer. The compositions of the polymer-blend solutions for electrospinning were varied for PEO molecular weight, total (alginate plus PEO) polymer concentration, and PEO proportion in the dry alginate PEO polymer mix used. These were tested for rheology (viscosity, complex viscosity, storage and loss moduli) and conductivity, and the electrospun nanofibers were characterized by scanning electron microscopy. One-parameter-at-a-time approach and response surface methodology (RSM) were used to optimize the polymer-blend solution composition to obtain defined nanofibers. Both approaches revealed that the major influence on nanofiber formation and diameter were total polymer concentration and PEO proportion. These polymer-blend solutions of appropriate conductivity and viscosity enabled fine-tuning of nanofiber diameter. PEO molecular weight of 2-4 million Da greatly improved the electrospinnability of alginate, producing nanofibers with >85% alginate. This study shows that RSM can be used to design nanofibers with optimal alginate and co-polymer contents to provide efficient scaffold material for regenerative medicine. nanofibers electrospinning alginate polyethylene oxide conductivity viscoelastic properties response surface methodology nanovlakna elektrospiniranje alginat polietilen oksid prevodnost viskoelastične lastnosti metodologija odzivne površine true false true Angleški jezik Slovenski jezik Članek v reviji 2021-03-12 10:58:28 2021-03-12 10:58:32 2022-09-05 03:50:22 0000-00-00 00:00:00 2019 0 0 20 str. iss. 4, art. 692 Vol. 11 Apr. 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2:620.3 2073-4360 10.3390/polym11040692 4704625 517951257 RAZ_Mirtic_Janja_2019.pdf RAZ_Mirtic_Janja_2019.pdf 1 8A5B257818028DAAB8C7EEF9708FB58D bbfdd1b4418cbadaf3964c79344b023c6504385dd4aa2943817cfedf40824a19 b21c0e80-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141053 https://www.mdpi.com/2073-4360/11/4/692 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141051 Fakulteta za farmacijo 0 0 0