20.500.12556/RUL-124554 Utilization of the zebrafish model to unravel the harmful effects of biomass burning during Amazonian wildfires Amazonian wildfires in 2019 have raised awareness about rainforest burning due to increased emissions of particulate matter and carbon. In the context of these emissions, by-products of lignin thermal degradation (i.e. methoxyphenols) are often neglected. Methoxyphenols entering the atmosphere may form intermediates with currently unknown reaction mechanisms and toxicity. This study for the first time provides a comprehensive insight into the impact of lignin degradation products [guaiacol, catechol], and their nitrated intermediates [4-nitrocatechol, 4,6-dinitroguaiacol, 5-nitroguaiacol] on zebrafish Danio rerio. Results revealed 4-nitrocatechol and catechol as the most toxic, followed by 4,6DNG > 5NG > GUA. The whole-organism bioassay integrated with molecular modeling emphasized the potential of methoxyphenols to inhibit tyrosinase, lipoxygenase, and carbonic anhydrase, consequently altering embryonic development (i.e. affected sensorial, skeletal, and physiological parameters, pigmentation formation failure, and non-hatching of larvae). The whole-organism bioassay integrated with in silico approach confirmed the harmful effects of lignin degradation products and their intermediates on aquatic organisms, emphasizing the need for their evaluation within ecotoxicity studies focused on aquatic compartments. ecology environmental sciences lignin degradation products ecotoxicity true false true Angleški jezik Angleški jezik Članek v reviji 2021-02-02 11:23:01 2021-02-02 11:23:02 2022-09-04 04:00:09 0000-00-00 00:00:00 2021 0 0 Str. 1-12 Vol. 11 2021 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 574:615.9 2045-2322 49719811 18727432 RAZ_Babic_Sanja_2021.pdf RAZ_Babic_Sanja_2021.pdf 1 7A136CAEA2B6ECB59886628F878367F5 5fad200480924632c8f717249f2b103ce8d14bbbd9d2494641160630c62fd2a2 94eca060-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=140077 https://www.nature.com/articles/s41598-021-81789-1 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=140076 Zdravstvena fakulteta 0 0 0