20.500.12556/RUL-122358 Ergot and ergot alkaloids in cereal grains intended for animal feeding collected in Slovenia occurrence, pattern and correlations This four-year study reports the occurrence of ergot alkaloids (EAs) in cereals intended for animal feeding collected in Slovenia. A total of 517 samples of cereals were analysed using liquid chromatography-tandem mass spectrometry for the presence of EAs. The sample set included wheat, rye, triticale, oat, spelt and barley. The study revealed that 17% of the analysed cereal samples were contaminated with at least one ergot alkaloid. EAs have two epimeric forms: -ine and -inine. The incidence rates of the -ine and -inine forms in the analysed samples were 16% and 15%, respectively. The highest contamination rates were observed in rye (54%), oat (50%) and spelt (30%), where the highest mean concentrations of total EAs were also determined (502 µg/kg, 594 µg/kg and 715 µg/kg, respectively). However, the highest concentrations of total EAs were found in wheat and rye (4217 µg/kg and 4114 µg/kg, respectively). The predominant EAs were ergometrine, ergosine and ergocristinine. The occurrence of six or more ergot alkaloids was observed in 49% of the positive samples. A weak correlation (p = 0.284) in the positive samples was found between the mass of sclerotia and the total concentrations of EAs using the Spearman correlation coefficient. animal feed analysis toxicity ergot alkaloid occurrence cereals LC-MS/MS correlation survey sclerotia alkaloids edible grain questionnaires true false true Angleški jezik Ni določen Članek v reviji 2020-12-03 09:40:10 2020-12-03 09:40:11 2022-09-02 04:02:56 0000-00-00 00:00:00 2020 0 0 18 str. iss. 11, art. 730 Vol. 12 2020 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 636.082:615.9:543:547 2072-6651 10.3390/toxins12110730 40459011 517594649 RAZ_Babic_Janja_2020.pdf RAZ_Babic_Janja_2020.pdf 1 24FBB67BF8240A63E87DFF84BAA7E3A9 20e40e6384d7b4bad216f5696f4037ea8fc4116466a1eed893c126c780234b94 bfd55461-8039-11ec-abdb-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=153364 https://www.mdpi.com/2072-6651/12/11/730 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=137543 Veterinarska fakulteta Biotehniška fakulteta 0 0 0