20.500.12556/RUL-122068 Nitrosative stress in the frontal cortex from dogs with canine cognitive dysfunction Canine cognitive dysfunction (CCD) is an age-related disorder similar to human Alzheimer's disease (AD) that occurs in elderly dogs. Nitrosative stress has been implicated as one of the causes leading to neurodegenerative diseases, particularly AD. Its involvement in the development of CCD has not been studied so far. In the present study, immunohistochemical staining detected all three isoforms of nitric oxide synthases (nNOS, eNOS, and iNOS) and 3-nitrotyrosine (3-NT) in brains from CCD-affected dogs and non-demented control dogs in all layers of the canine frontal cortex. In CCD-affected and non-demented brains, nNOS was highly expressed in pyramidal-like neurons in the upper cortical layers. nNOS has also been observed in astrocytes in the CCD frontal cortex. The nNOS immunohistochemical staining was statistically significantly elevated in dogs with CCD in comparison to non-demented dogs. Blood vessel wall cells were positive for eNOS, which was also expressed in astrocytes and neurons. Intense 3-NT immunoreactivity was observed in the upper cortical layers, where amyloid-beta deposits spread in the last stage of CCD. Brain cells in the same area were highly immunoreactive for iNOS. This infers that neuroinflammation and nitrosative stress might exacerbate the neurodegenerative process in CCD-affected brains, ultimately leading to cognitive impairment. canine cognitive dysfunction nitrosative stress nNOS eNOS iNOS 3-nitrotyrosine cognitive dysfunction brain dogs pasja kognitivna motnja nitrozativni stres nNOS eNOS iNOS 3-nitrotirozin true false true Angleški jezik Slovenski jezik Članek v reviji 2020-11-19 13:04:58 2020-11-19 13:04:59 2025-04-23 09:43:40 0000-00-00 00:00:00 2020 0 0 14 str. Vol. 7, art. 573155 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.7.09:616.89 2297-1769 10.3389/fvets.2020.573155 38304259 3969402 fvets-07-573155_(2).pdf fvets-07-573155_(2).pdf 1 45DEA5CDF46E343909E8D0711B66B4FD f722789f5ee7fd8a5c8fba0a128d803a5bf0c61b9d257969ef9c7af055eb8443 cb13424e-1b6d-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=208648 https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2020.573155/full 0 e988bf6e-1f81-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=209649 Veterinarska fakulteta Fakulteta za kemijo in kemijsko tehnologijo 0 0 0