20.500.12556/RUL-119702 Sinteza bifunkcionalnih ročic za vezavo tiolov Synthesis of bifunctional compounds for binding of thiols V svojem diplomskem delu opisujem sintezo treh heterobifunkcionalnih ročic za čiščenje proteinov z afinitetno kromatografijo, ki imajo na koncih N-hidroksisukcinimidno in bromoacetilno skupino. Izhajal sem iz bromoacetil bromida, ki sem ga modificiral s 3-aminopropanojsko oz. 6-aminoheksanojsko kislino. Iz bromoacetil bromida in njegovih derivatov sem z NHS tvoril funkcionalizirane bifunkcionalne ročice. Sintezi ročic z najkrajšo in najdaljšo verigo sta bili uspešni, ročice s srednje dolgo verigo pa mi ni uspelo pripraviti. In this diploma work, the syntheses of three heterobifunctional crosslinkers for isolation of proteins with affinity chromatography that have N-hydroxysuccinimide ester and bromoacetyl groups on their ends are described. Bromoacetyl bromide was transformed to desired bromoacetyl-functionalized carboxylic acid using 3-aminopropanoic acid and 6-aminopropanoic acid. NHS-functionalized bifunctional crosslinkers were prepared from bromoacetyl bromide and its derivatives by activation of carboxylic acid and esterification with N-hydroxysuccinimide. The syntheses of crosslinkers with the shortest and longest chains were succesfull whereas the synthesis of the crosslinker with medium lenght chain was unsuccessful. ročica monolit bifunkcionalno afinitetna kromatografija crosslinker monolith bifunctional affinity chromatography true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2020-09-10 17:55:00 2020-09-10 17:55:01 2022-08-31 03:54:21 0000-00-00 00:00:00 2020 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 11943 30817795 Kisovec_Blaz_-_Sinteza_bifunkcionalnih_rocic_za_vezavo_tiolov.pdf Kisovec_Blaz_-_Sinteza_bifunkcionalnih_rocic_za_vezavo_tiolov.pdf 1 CC81F2FF5DDFAD05259445B93F282FBC e92d606e9ce653d4bb42e07ea2bea7d28ea24d3145e0e86e599ad7b6fb9b2749 e5fa6d94-a1b9-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=134427 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0