20.500.12556/RUL-119549 Kromatografija virusov in virusom podobnih delcev Chromatography of viruses and virus-like particles Kromatografija je laboratorijska tehnika pri kateri ločujemo posamezne komponente zmesi glede na njihovo separacijsko hitrost. Zmes je raztopljena v tako imenovani mobilni fazi, ki nato potuje skozi stacionarno fazo. Določevanje identitete in koncentracije molekul v zmesi izvajamo pri analitični kromatografiji. Preparativna kromatografija pa se uporablja za pripravo večjih količin čistih snovi posamezne molekulske vrste, ki jih lahko uporabimo kasneje. Virusi in virusom podobni delci (molekule, ki spominjajo na viruse, vendar so ne infektivne, ker ne vsebujejo genetskega materiala) ter bakteriofagi spadajo med nanoplekse in obdelava le teh je odvisna od kompleksnosti začetnega materiala, števila načinov očiščenja med katerimi izbiramo ter kriterijev, katerim mora končni produkt slediti. Prečiščeni virusni pripravki se že dolgo uporabljajo kot cepiva in imajo v prihodnosti veliki potencial v genski terapiji. Bakterijski virusi oziroma bakteriofagi že dokazano lahko zdravijo bakterijske okužbe, vendar je odkrivanje antibiotikov upočasnilo raziskavo v zvezi z njihovo uporabo. Zaradi večje odpornosti bakterij na antibiotike pa so spet prepoznani kot alternativni vir zdravljenja bakterijskih okužb. Prav zaradi tega bomo v naši raziskavi preučili možnost uporabe kromatografije za izolacijo virusov. Chromatography is a laboratory technique where individual components of a mixture are separated according to their separation rate. The mixture is dissolved in the so-called mobile phase, which then travels through the stationary phase. The determination of the identity and concentration of molecules in the mixture is performed by analytical chromatography. Preparative chromatography, however, is used to prepare large quantities of pure substances of each molecular species that can be used later. Viruses and virus-like particles (molecules that resemble viruses but are non-infectious because they do not contain genetic material) and bacteriophages are nanoplexes and their processing depends on the complexity of the starting material, the number of purification methods to choose from, and the criteria which the end product has to match. Purified viral preparations have already been used as vaccines and have potential in gene therapy. Bacterial viruses or bacteriophages have already been proven to treat bacterial infections, but antibiotics have slowed researches regarding them. Due to the higher resistance of bacteria to antibiotics, they are again recognized as an alternative source of treatment for bacterial infections. It is for this reason that our study will examine the use of chromatography for virus isolation. virus kromatografija virusu podoben delec virus chromatography virus like particle true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2020-09-09 17:45:00 2020-09-09 17:45:06 2022-08-31 03:53:37 0000-00-00 00:00:00 2020 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 11923 29649667 Kavcic_Neja_-_Kromatografija_virusov_in_virusom_podobnih_delcev.pdf Kavcic_Neja_-_Kromatografija_virusov_in_virusom_podobnih_delcev.pdf 1 D310A6A564342207192FD35BEA1866A9 8b6a7454b64dcd5555635361d70ac51cfe2b61e8330b586ca2e48ab1c0833841 dc1b023b-a1b9-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=134274 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0