20.500.12556/RUL-119346 Uporaba raziskovalnega invertnega mikroskopa za fluorescenčno termometrijo Use of research inverted microscope for fluorescence thermometry V delu obravnavamo uporabo raziskovalnega invertnega mikroskopa Nikon Eclipse Ti2 za fluorescenčno termometrijo. V okviru testiranja invertnega mikroskopa za uporabo za fluorescenčno termometrijo smo izvedli eksperiment merjenje fluorescence vzorcev. Merilni sistem je zastavljen tako, da omogoča hkratno uporabo optične pincete in dveh kamer z globalno in vrtečo zaslonko. Na primeru spektra fluorescenčnih vzorcev smo prikazali delovanje invertnega mikroskopa, vzbujevalnega LED svetila, tekočekristalnega filtra in kamere z vrtečo zaslonko in CMOS senzorjem. This work discusses the use of the Nikon Eclipse Ti2 research inverted microscope for fluorescence thermometry. As part of the testing of the inverted microscope for use in fluorescence thermometry, we performed an experiment measuring the fluorescence of samples. The system is designed to allow the simultaneous use of optical tweezers and two cameras with global and rolling shutter. In the case of the spectrum of fluorescent samples, we showed the operation of an inverted microscope, an excitation LED lamp, a liquid crystal filter and a rolling shutter camera with CMOS sensor. fluorescenca fluorescenčna termometrija invertni mikroskop meritve spektra fluorescence fluorescence fluorescence thermometry inverted microscope fluorescence specter mesurments true false false [J. Mušič] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2020-09-08 08:32:13 2020-09-08 08:32:14 2022-08-31 03:52:39 0000-00-00 00:00:00 2020 0 Ljubljana 0 XXII, 52 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 519.6:536.51(043.2) 223717 32531715 5880.pdf 5880.pdf 1 5512CAC6F2AC17F520D34A2358CEA53D 988765ef920a96abbc652960dd876adcb52f70f4aa399dc56b48b7db4095b5f7 cd35c9f9-a1b9-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=134046 Fakulteta za strojništvo 0 0 0