20.500.12556/RUL-118011 Proteomic analysis of fresh and liquid-stored boar spermatozoa In this study comparative proteomics was used to define changes in the expression of the spermatozoa proteins during liquid storage. Semen from eight boars was analyzed on the day of collection and after liquid preservation at 15–17 °C for three days. Sperm parameters (concentration, motility, morphology, vitality) and percentage of non-capacitated and acrosomal-reacted spermatozoa were determined. Sperm proteins were extracted and separated by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and proteomic profiles were computationally compared to highlight differentially expressed protein spots that were, in turn, identified by mass spectrometry. The intensities of four spots were significantly different between fresh and liquid stored sperm. Namely: ATP citrate lyase, chaperonin containing T-complex polypeptide 1 (TCP1) subunit ε and probable phospholipid-transporting ATP-ase were over-expressed in liquid stored sperm, whereas cytosolic non-specific dipeptidase was over-expressed in fresh sperm. These differentially expressed proteins could be used as plausible biomarkers for the evaluation of boar semen quality and spermatozoa survival after liquid storage and could help to address problems associated with sperm preservation. proteomics spermatozoa metabolism semen analysis semen preservation methods liquid stored semen boar spermatozoa swine true false true Angleški jezik Ni določen Članek v reviji 2020-08-11 10:47:06 2020-08-11 10:47:11 2022-08-30 04:01:01 0000-00-00 00:00:00 2020 0 0 10 str. iss. 4, art. 553 Vol. 10 Apr. 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4.082.453.5:612.2:591.463.1:542.816 2076-2615 10.3390/ani10040553 5144442 519120409 RAZ_Premrov_Bajuk_Blanka_2020.pdf RAZ_Premrov_Bajuk_Blanka_2020.pdf 1 57B7C09A23F7ABAA60C09D7BBC4474BD f00a70bdc044cd15bf7a57dd4936a74c5f9679af6ae1fef3ebb706e2f9a78e66 bc793683-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141337 https://www.mdpi.com/2076-2615/10/4/553 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=131944 Veterinarska fakulteta 0 0 0