20.500.12556/RUL-116159 The interaction between the osteosarcoma cell and stainless steel surface, modified by high-fluence, nanosecond laser pulses The irradiation of metallic surfaces by high-fluence laser pulses in an oxygen-containing atmosphere inevitably modifies the surface topography, chemistry, and wettability. These modifications significantly influence cell-surface interactions and, consequently, surface biocompatibility. We investigate how surface texturing by high-fluence nanosecond laser pulses from a Nd:YAG laser (wavelength of 1064 nm) influences cell adhesion and morphology with the aim of assessing its impact on initial cell behaviour. Quantitative and qualitative analysis of osteosarcoma cell adhesion, viability, and cell morphology were evaluated after 24-hour exposure to non-treated and laser-textured stainless-steel (AISI 316L) surfaces by fluorescent and scanning electron microscopy. The results reveal that this, initial interaction between the cells and the laser-textured surfaces leads to round shaped cells with a smaller footprint. Contrarily, on the non-processed stainless-steel and control-glass surfaces the polygonal, highly elongated, and flattened cells are observed. The cells on the laser-textured surfaces are less dendritic, with short tubular protrusions and an overexpression of extracellular vesicles, which are rarely found on non-treated and control samples. This likely happens due to the formation of nanostructured, high-temperature oxides that are induced by laser ablation. The analysis by X-ray photoelectron spectroscopy reveals that the laser-textured stainless-steel surfaces contain Cr hexavalent oxide, which is more toxic than the native oxide layer on the non-processed samples. laser surface engineering laser processing biocompatibility biological cell nanosecond-laser ablation laser texturing MG63 cells cell adhesion laserski inženiring površin laserske obdelave biokompatibilnost biološke celice nanosekundnolaserska ablacija lasersko teksturiranje celice MG63 adhezija celic true false true Angleški jezik Slovenski jezik Članek v reviji 2020-05-20 08:21:23 2020-05-20 08:21:31 2022-08-28 03:47:26 0000-00-00 00:00:00 2020 0 0 12 str. art. 125878 Vol. 394 May 2020 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 621.7.015(045) 0257-8972 10.1016/j.surfcoat.2020.125878 14290947 3084815 RAZ_Hocevar_Matej_2020.pdf RAZ_Hocevar_Matej_2020.pdf 1 E8AD4DA31A3FA3B60E10F7C110C514AD c5d6d2a6c3d106f3fd5e9b7ed031408893255f94bee2448f752ef3f94a3d255c c172ff66-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141453 RAZ_Hocevar_Matej_2020.pdf RAZ_Hocevar_Matej_2020.pdf 1 E039E56BB9B42D082D680C63646D15FB 741e7307b4d43db84a80c68281495085e4621c6a6ebb85184b485152e4b0690c c183b966-a1bb-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=141454 https://www.sciencedirect.com/science/article/pii/S0257897220305478 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=129569 Biotehniška fakulteta Fakulteta za strojništvo 0 0 0