20.500.12556/RUL-114973 Primerjava prehranskih dopolnil iz alg: antioksidativni potencial in izotopska sestava Comparison of algae dietary supplements: antioxidative potencial and izotopic composition Antioksidativni potencial in izvor prehranskih dopolnil na osnovi alg sta slabo znana, saj prehranska dopolnila nimajo stroge regulacije tako kot zdravila in živila. Z določanjem antioksidativnega potenciala - AOP (metoda DPPH in Folin-Ciocalteu), elementne sestave (XRF in ICP-MS) in izotopske sestave lahkih elementov (EAIRMS), smo medsebojno ovrednotili posamezne vzorce prehranskih dopolnil na osnovi Aphanizomenon flos-aquae - AFA, Spirulina sp., Chlorella sp. in rjavih alg. AFA imajo 4,4 krat višji AOP v primerjavi s Chlorella sp. in 2,7 krat višji v primerjavi s Spirulina sp., medtem ko imajo rjave alge zanemarljiv AOP. Vsebnosti elementov v priporočenih odmerkih PD ne presegajo mejnih vrednosti za vnos esencialnih elementov. Koncentracija toksičnih elementov (arzen, kadmij, živo srebro in svinec) v vzorcih je nizka in posledično ne predstavlja tveganja za zdravje potrošnikov. Nekateri vzorci imajo bistveno povišano vsebnost silicija, kar nakazuje na dodatek nedeklariranega SiO2. S pomočjo statistične obdelave (analiza glavnih komponent in analiza razvrščanja enot v skupine) rezultatov analiz, lahko potrdimo enoten izvor AFA, ki naj bi bilo jezero Klamath. Havajsko S. pacifica je tudi možno ločiti od ostalih vzorcev predvsem zaradi značilne elementne sestave. Zaradi različnih izotopskih razmerij stabilnih elementov (C, N in S) v vzorcih Spirulina sp. in Chlorella sp. predvidevamo, da proizvajalci uporabljajo hranila različnega izvora in različne tehnike gojenja alg. Antioxidative potential and origin of algae based food supplements are relatively unknown, as food supplements do not have strict regulation compared to food and medicinal drugs. Our goal was to evaluate different samples of food supplements that are based on Aphanizomenon flos-aquae – AFA, Spirulina sp., Chlorella sp. and brown algae, by determining antioxidative potencial - AOP (methods DPPH and Folin-Ciocalteu), elemental composition analysis (XRF and ICP-MS) and ratios of stables isotopes of light elements (C, N and S; EA-IRMS method). AFA samples have 4.4 higher AOP compared to Chlorella sp. and 2,7 higher compared to Spirulina sp., while AOP in brown algae is negligible. Manufacturer's suggested doses of samples do not exceed upper intake level of essencial elements. Concentrations of toxic elements (arsenic, cadmium, mercury and lead) are low and do not represent risk to consumer's health. Few samples have increased silicon content, which could mean addition of undeclared additive SiO2. Statistical analysis (principal component analysis and cluster analysis) has confirmed that AFA samples originate from the same source, which is supposedly lake Klamath. Hawaiian S. pacifica can also be differentiated from other samples, due to characteristic elemental composition. Different isotopic ratios of stable elements (C, N and S) of Spirulina sp. and Chlorella sp. samples are consequence of using different nutrient sources and algae growing techniques. prehrana prehranska dopolnila alge Spirulina sp. Chlorella sp. klamatske alge rjave alge elementna sestava izotopska sestava antioksidativni potencial fenolne spojine analitske metode DPPH Folin-Ciocalteu food food supplements algae Spirulina sp. Chlorella sp. Klamath algae kelp elemental composition isotopic composition antioxidative potential phenolic compounds analytical methods DPPH Folin-Ciocalteu true false false [J. Kejžar] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2020-04-05 07:15:09 2020-04-05 07:15:18 2022-08-27 03:58:57 0000-00-00 00:00:00 2020 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.232:641:665.939.35:543.51 175755 5180792 9757.pdf 9757.pdf 1 6972517C34B2A3845F0390DDD1218348 3a3124a951caae373e5ad91989d8e7a82804ddc00060e36f5c643ed9a9d90a30 552a6aa3-a1b8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=127455 Biotehniška fakulteta 0 0 0