20.500.12556/RUL-113787 Evaluation of human milk microbiota by 16S rRNA gene next-generation sequencing (NGS) and cultivation/MALDI-TOF mass spectrometry identification The aim of the present study was to characterize human milk microbiota (HMM) with 16S rRNA gene amplicon next-generation sequencing and cultivation/matrix-assisted laser desorption/ionization (MALDI)-time of flight (TOF) mass spectrometry (MS) identification approaches. We analyzed 31 human milk samples from healthy Slovenian mothers. To check the accuracy of MALDI-TOF MS identification, several colonies representing most abundant genera and those, which could not be reliably identified by MALDITOF, were subjected to Sanger sequencing of their 16S rRNA gene. We showed that cultivation/MALDI-TOF MS was a suitable tool for culture-dependent determination of HMM. With both approaches, Staphylococcus and Streptococcus were found as predominant genera in HMM and the abundance of Staphylococcus was associated with decreased microbial diversity. In addition, we characterized factors that might influence HMM. The use of a breast pump was significantly associated with composition of HMM, higher microbial load, and lower abundance of cultivable staphylococci. Moreover, our study suggests that administration of probiotics to the suckling infant might influence HMM by increased abundance of lactobacilli and the presence of viable probiotic bacteria in human milk. However, since our study was observational with relatively small sample size, more targeted studies are needed to study possible transfer of probiotics to the mammary gland via an external route and the physiological relevance of these events. milk microbiota microbiota assembly breastfeeding breast pump probiotic drops mikrobiologija humano mleko mikrobiota genetika masna spektrometrija true false true Angleški jezik Slovenski jezik Članek v reviji 2020-02-04 14:51:43 2020-02-04 14:51:45 2023-05-04 12:59:00 0000-00-00 00:00:00 2019 0 0 12 str. November, art. 2612 Vol. 10 2019 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 579 1664-302X 10.3389/fmicb.2019.02612 4326792 4146296 RAZ_Treven_Primoz_2019.pdf RAZ_Treven_Primoz_2019.pdf 1 A6540B233BCEE421244D03033F6CCE23 19c65c05bb2364d79f357540c426ef81aac6b084b3af2ee63cea73add9bf20b5 3cc0c8b8-a1ba-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=136017 RAZ_Treven_Primoz_2019.pdf RAZ_Treven_Primoz_2019.pdf Corrigendum Corrigendum 1 4B221EF9DD19CB69019AEA74BEFF3E30 d713459271c064cf5e93e6a422490d703acd43f650c322dcfe085ce2da4c71df 2ee4d1e1-f516-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145723 https://www.frontiersin.org/articles/10.3389/fmicb.2019.02612/full 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=136016 https://www.frontiersin.org/articles/10.3389/fmicb.2020.00031/full Corrigendum Corrigendum 0 530f6f43-f516-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=145724 Biotehniška fakulteta Veterinarska fakulteta 0 0 0