20.500.12556/RUL-113472 Fungal and bacterial diversity of Svalbard subglacial ice The composition of fungal and bacterial communities in three polythermal glaciers and associated aquatic environments in Kongsfjorden, Svalbard was analysed using a combination of cultivation and amplicon sequencing. 109 fungal strains belonging to 30 mostly basidiomycetous species were isolated from glacial samples with counts up to 10$^3$ CFU/100 ml. Glaciozyma-related taxon and Phenoliferia psychrophenolica were the dominant species. Unexpectedly, amplicon sequencing uncovered sequences of Chytridiomycota in all samples and Rozellomycota in sea water, lake water, and tap water. Sequences of Malassezia restricta and of the extremely halotolerant Hortaea werneckii were also found in subglacial habitats for the first time. Overall, the fungal communities within a glacier and among glaciers were diverse and spatially heterogenous. Contrary to this, there was a large overlap between the bacterial communities of different glaciers, with Flavobacterium sp. being the most frequently isolated. In amplicon sequencing Actinobacteria and Proteobacteria sequences were the most abundant. fungi bacteria ice Svalbard true false true Angleški jezik Ni določen Članek v reviji 2020-01-09 15:24:51 2020-01-09 15:24:52 2022-08-25 03:56:32 0000-00-00 00:00:00 2019 0 0 15 str. Vol. 9, art. 20230 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579 2045-2322 10.1038/s41598-019-56290-5 5274703 RAZ_Perini_Laura_2019.pdf RAZ_Perini_Laura_2019.pdf 1 ADE469B18141A5C2BB02671822136618 b94f56a257727a313eee39965d73bce62fc036ec31c4712ca2d0eb566552b495 1d24c1dc-a1b8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=125563 https://www.nature.com/articles/s41598-019-56290-5 0 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=125562 Biotehniška fakulteta 0 0 0