20.500.12556/RUL-113295 Razvoj metode za ugotavljanje znotrajceličnih koncentracij izbranih zaviralcev imunoproteasoma psolarenskega tipa na osnovi tekočinske kromatografije ultra visoke ločljivosti Development of an ultra-high-performance liquid chromatography method for determination of intracellular concentrations of selected psolaren-based immunoproteasome inhibitors Immunoproteasom je znotrajcelična proteaza, katere glavna fiziološka funkcija je, da razgrajuje nepravilno zvite in poškodovane proteine. Zaviranje proteolitične aktivnosti imunoproteasoma bi lahko bilo ugodno pri zdravljenju nekaterih rakavih obolenj oz. avtoimunih bolezni. Izbrani zaviralci imunoproteasoma niso izkazovali predvidenega učinka na celični liniji, za kar je lahko kriva premajhna znotrajcelična koncentracija zaviralca. Izbrani zaviralci so namreč zelo lipofilni in ker gre za kovalentne zaviralce, lahko tudi reagirajo s proteini v celičnem mediju. V magistrski nalogi bomo poizkusili razviti metodo na osnovi UHPLC za ugotavljanje intracelularne koncentracije izbranih zaviralcev. Immunoproteasome is an intracellular protease, whose pivotal role is degradation of abnormally folded and damaged proteins. Inhibition of the proteolytic activity of the immunoproteasome could be advantageous in the treatment of certain cancers and autoimmune diseases. The selected immunoproteasome inhibitors did not show the intended effect on the cell line, which may be due to the insufficient intracellular inhibitor concentration. The selected inhibitors are very lipophilic and because they are covalent inhibitors, they can also react with proteins in the cell medium. In the master's thesis we will try to develop a method based on UHPLC to determine the intracellular concentration of selected inhibitors. Imunoproteasom kovalentni zaviralci tekočinska kromatografija ultra visoke ločljivosti LR057 GBB102 Immunoproteasome covalent inhibitors ultra-high-performance liquid chromatography LR057 GBB102. true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2019-12-19 08:35:09 2019-12-19 08:35:12 2022-08-25 03:55:42 0000-00-00 00:00:00 2019 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 72668 819.pdf 819.pdf 1 00E7F9C1E228BEC0AFF02BBF98074904 c7b00fc06d00d2e910d1d43a0451aff24e61783931b38605da9c77e03aea5652 114285ac-a1b8-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=125342 Fakulteta za farmacijo 0 0 0