20.500.12556/RUL-113283 Management of a Coxiella burnetii-infected sheep flock after an outbreak of Q fever in humans Following an outbreak of Q fever in a group of students who contracted the infection during a training course on a sheep farm, a detailed investigation of the sheep flock involved was conducted. Of 478 flock animals, 60 Coxiella burnetii ELISA-positive and 60 ELISA-negative ewes were selected for the trial and divided into four groups. A month after the initial ELISA screening, all ewes in the flock (except the control group) were vaccinated. Sequentially collected blood samples were tested with ELISA and PCR; feces, milk, manure, bedding, and soil were tested with PCR. The immune response to the vaccination was 92.7%, while the overall C. burnetiiseroprevalence in the flock after the human outbreak was 64.9%. PCR was positive for 0.2% of milk samples and 34.4% of fecal samples of animals from all four trial groups. C. burnetii DNA was not detected in any of the blood samples. Manure was PCR-positive for about 35 months; bedding from the stable was also positive while samples of pasture soil were negative. It appears that extensive cleaning and disinfection combined with vaccination could be regarded as an appropriate approach to control/prevent Q fever in farm settings even in the short term. Coxiella burnetii Q fever prevention and control sheep vaccination serology environment true false true Angleški jezik Ni določen Članek v reviji 2019-12-18 12:47:00 2019-12-18 12:47:01 2025-05-08 12:09:03 0000-00-00 00:00:00 2019 0 0 Str. 264-270 no. 2 Vol. 43 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.9 1300-0128 10.3906/vet-1806-31 4786554 512343065 Management_of_a_Coxiella_burnetii-infected_sheep_flock_after_an_o.pdf Management_of_a_Coxiella_burnetii-infected_sheep_flock_after_an_o.pdf 1 11CBDBAE20609CE0E411F4E25FB02D05 97d61f5db98da9c3e656ba02062c1554bf2d79eee610d2ae556a588c8353c9ec 332e78ea-1acc-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=208536 https://journals.tubitak.gov.tr/veterinary/vol43/iss2/12/ 0 05e2c3a6-2bf1-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210171 Veterinarska fakulteta 0 0 0