20.500.12556/RUL-111832 Viscoelastic response of Escherichia coli biofilms to genetically altered expression of extracellular matrix components How viscoelastic properties of the extracellular matrix affect the various biological functions conferred by biofilms is an important question in microbiology. In this study viscoelastic response of Escherichia coli biofilms to genetically altered expression of extracellular matrix components was studied. Biofilms of the wild type E. coli MG1655 and its mutant strains producing different amounts of extracellular matrix components (curli, colonic acid, poly-β-1,6-N-acetyl-D-glucosamine) were used to examine the viscoelastic behavior of biofilms grown at solid -atmosphere interface. The results suggest that the presence of curli proteins dominates biofilm mechanical behavior. The rheological data indicate that cohesive energy of the biofilm was highest in the wild type strain. The results demonstrate the importance of extracellular matrix composition for biofilm mechanical properties. We propose that by genetically altering the expression of extracellular matrix polymers bacteria are able to modulate the mechanical properties of their local environment in accordance with bulk environmental conditions. biofilm Escherichia coli EPS rheology viscoelasticity biofilmi Escherichia coli zunajcelični polimeri reološke lastnosti viskoelastičnost true false false Angleški jezik Slovenski jezik Znanstveno delo 2019-10-15 09:46:35 2019-10-15 10:08:06 2022-08-24 03:47:27 0000-00-00 00:00:00 2019 0 0 Str. 5042-5051 Vol. 15 2019 0000-00-00 PostprintKoncna NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2020-05-28 579.24/.26:579.842:544 1744-683X 10.1039/c9sm00297a 5060728 19616551 Stopar_soft_matter_2019.pdf Stopar_soft_matter_2019.pdf 1 285874CC898F018A1287D20935E648C6 2f80f235e79e3434010c1a100b2a02fdafe126e350817fb1c0d5c35e17f0dab7 82b12788-a1b7-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=123861 https://pubs.rsc.org/en/content/articlelanding/2019/SM/C9SM00297A#!divAbstract 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=123739 Biotehniška fakulteta 0 0 0