20.500.12556/RUL-111352 Mikropropagacija gorenjskega nageljna (Dianthus sp. L.) Micropropagation of Gorenjski carnation (Dianthus sp. L.) V in vitro poskus smo vključili 6 rastlin gorenjskega nageljna (Dianthus sp.) iz katerih smo pripravili nodijske in listne izsečke ter jih razkužili z 2 % raztopino dikloroizocianurne kisline. Nodijske izsečke smo inokulirali na osnovno MS gojišče z dodatkom BAP in NAA (3 in 0,5 mg/l) - gojišče 1 ter enako koncentracijo BAP in NAA (1 mg/l) - gojišče 2. Listne izsečke smo inokulirali prav tako na osnovno MS gojišče z dodatkom BAP (2,5 mg/l) - gojišče 3 in gojišče 4 z BAP (5 mg/l). Na gojišču 1 je bila regeneracija 38,7 % in na gojišču 2 je bila za 6,3 % boljša (45 %). Na gojišču 1 smo zaradi okužbe izgubili 13,1 % izsečkov in poganjkov ter na gojišču 2 je bilo okuženih samo 7,7 % izsečkov in poganjkov. Nastale poganjke smo subkultivirali na gojišče za koreninjenje 5 z IBA (5 mg/l) in 24 % poganjkov se je uspešno koreninilo. Z namenom izboljšati fazo koreninjenja smo 135 poganjkov subkultivirali na gojišče za koreninjenje 6 z IAA (2,5 mg/l) in kar 45,8 % poganjkov je oblikovalo korenine. Koreninjeni poganjki so bili vitalni in primerni za aklimatizacijo. V prihodnje bi lahko preko meristemske in vitro kulture pridobivali brezvirusne sadike gorenjskega nageljna. We included 6 samples of Dianthus sp. in the in vitro experiment. From them we prepared leaf and node explants and disinfected them using a 2 % solution of dichloroisocyanuric acid. We inoculated node explants on a basic Murashige and Skoog’s (MS) growth medium enriched with BAP and NAA. The first medium was supplemented with 3 mg/l BAP and 0.5 mg/l NAA and the second included equimolar concentrations of both hormones (1 mg/l). Furthermore, we inoculated leaf explants on the same MS medium with added BAP in two different concentrations. 2.5 mg/l was added to medium 3 and 5 mg/l to medium 4. Growth medium 2 gave the highest regeneration response followed by medium 1 (45 % and 38.7 % respectively). There was a 13.1 % loss of shoot buds and explants due to infection on medium 1 and 7.7 % on medium 2. Newly formed adventitious shoot buds were subcultured on a root formation medium with IBA (5 mg/l). 24 % of shoot buds were successfully rooted in. We subcultured 135 shoots on a root formation medium with IAA (2.5 mg/l) with the purpose of improving root formation. As a result of this procedure 45.8 % shoot buds formed viable roots suitable for acclimatisation. Henceforth a possibility may arise to acquire virus – free saplings of Dianthus sp. through meristem in vitro cultures. in vitro kultura gorenjski nagelj Diatnhus sp. genotip nodijski izsečki meristem listni izsečki gojišče hormoni regeneracija koreninjenje in vitro culture carnation Diatnhus sp. genotype nodal explants meristem leaf explants culture medium hormones regeneration rooting true false false [Ž. Doljak] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-09-28 07:45:25 2019-09-28 07:45:27 2022-08-23 03:42:30 0000-00-00 00:00:00 2019 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.661.1:57.082.2(043.2) 171377 9314425 8411.pdf 8411.pdf 1 B51290D268EB0633BCC42D04D466C84A 091b68fd803cd56d0b7ef8fc50e4a60069b66fb932921d693ce58237fc30694a 5d8bd160-a1b7-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=123189 Biotehniška fakulteta 0 0 0