20.500.12556/RUL-109393 Antioksidativni potencial konopljinih semen Antioxidant potential of hemp seeds Konopljina semena, katerih priljubljenost vse bolj narašča, so izvrsten vir hranilnih snovi, esencialnih aminokislin in maščobnih kislin. Vsebujejo tudi nekatere bioaktivne molekule, ki delujejo kot antioksidanti in imajo pozitiven vpliv na zdravje človeka. Cilj diplomske naloge je bil določiti vsebnost fenolnih spojin v različnih sortah konopljinih semen. Prav tako smo določali antioksidativni potencial (AOP) konopljinih semen tako v homogenem (etanol), kot tudi heterogenem (emulzija linolne kisline v vodi) okolju. Semena smo zmleli in razmastili ter ekstrahirali fenolne spojine s 70 % etanolom. Določali smo skupne fenolne spojine s Folin Ciocalteujevo metodo, sposobnost lovljenja prostih radikalov DPPH• in izvedli metodo beljenja ?-karotena v emulziji. Ugotovili smo, da se učinkovitost posameznih antioksidantov razlikuje v različnih sistemih. Prav tako smo prišli do zaključka, da sorte z največ fenolnih spojin nimajo največjega AOP. Hemp seeds are an excellent source of nutrients, such as essential amino acids and fatty acids, and their popularity is growing every day. They also contain bioactive molecules that function as antioxidants and therefore have a positive impact on human health. The aim of graduation thesis was to determine content of total phenolic compounds in different sorts of hemp seeds. We have also examined antioxidant potential (AOP) in different systems: homogeneous (ethanol) and heterogeneous (emulsion of linoleic acid in water) system. Before the extraction of phenolic compounds in 70 % ethanol, hemp seeds were grinded and defatted. We determined total phenolic content with Folin Ciocalteu method, analysed the scavenging activity of free DPPH• radicals and carried out ?-carotene bleaching assay in emulsion. We came to the conclusion that activity of particular antioxidant in homogeneous and heterogeneous system differentiates. Furthermore, hemp seeds with the highest content of phenolic compounds do not have the highest AOP. industrijska konoplja Cannabis sativa L. konopljina semena antioksidanti fenolne spojine antioksidativni potencial industrial hemp Cannabis sativa L. hempseed antioxidants phenolic compounds antioxidant potential true false false [U. Šetina] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-09-01 07:45:03 2019-09-01 07:45:08 2022-08-21 03:41:48 0000-00-00 00:00:00 2019 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.522:66.094.3.097.8:547.56 166914 5087352 7556.pdf 7556.pdf 1 AB3C9F131CCB22145204C7E45D166576 4e1a03be7f42fcc5a4269998c1843259b58d495be470aefb759548d517343f4f d959a453-a1b6-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=120932 Biotehniška fakulteta 0 0 0