20.500.12556/RUL-108955 Antimicrobial resistance and virulence genes in Enterococcus faecium and Enterococcus faecalis from humans and retail red meat The emergence of antimicrobial-resistant and virulent enterococci is a major public health concern. While enterococci are commonly found in food of animal origin, the knowledge on their zoonotic potential is limited. The aim of this study was to determine and compare the antimicrobial susceptibility and virulence traits of Enterococcus faecalis and Enterococcus faecium isolates from human clinical specimens and retail red meat in Slovenia. A total of 242 isolates were investigated: 101 from humans (71 E. faecalis, 30 E. faecium) and 141 from fresh beef and pork (120 E. faecalis, 21 E. faecium). The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of seven common virulence genes was investigated using PCR. In both species, the distribution of several resistance phenotypes and virulence genes was disparate for isolates of different origin. All isolates were susceptible to daptomycin, linezolid, teicoplanin, and vancomycin. In both species, the susceptibility to antimicrobials was strongly associated with a food origin and the multidrug resistance, observed in 29.6% of E. faecalis and 73.3% E. faecium clinical isolates, with a clinical origin (Fisher’s exact test). Among meat isolates, in total 66.0% of E. faecalis and E. faecium isolates were susceptible to all antimicrobials tested and 32.6% were resistant to either one or two antimicrobials. In E. faecalis, several virulence genes were significantly associated with a clinical origin; the most common (31.0%) gene pattern included all the tested genes except hyl. In meat isolates, the virulence genes were detected in E. faecalis only and the most common pattern included ace, efaA, and gelE (32.5%), of which gelE showed a statistically significant association with a clinical origin. These results emphasize the importance of E. faecalis in red meat as a reservoir of virulence genes involved in its persistence and human infections with reported severe outcomes. antibacterial agents bacteria Enterococcus faecalis Enterococcus faecium pharmacology drug resistance genetics drug effects growth development virulence factors meat microbiology vancomycin resistance models animals veterinary drugs humans red meat Slovenia true false true Angleški jezik Ni določen Članek v reviji 2019-08-13 13:53:24 2019-08-13 13:53:25 2025-04-23 14:17:48 0000-00-00 00:00:00 2019 0 0 12 str. art. 2815279 Vol. 2019 2019 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.09:597 2314-6141 10.1155/2019/2815279 4816506 30624729 BioMed_Research_International_-_2019_-_Golob_-_Antimicrobial_Resistance_and_Virulence_Genes_in_Enterococcus_faecium_and.pdf BioMed_Research_International_-_2019_-_Golob_-_Antimicrobial_Resistance_and_Virulence_Genes_in_Enterococcus_faecium_and.pdf 1 5BB6B7F0DB1CA1B9A318B622417FD15B 51c2ab696962bb87dfe2668eaebd074659c6bd3c701fbbd0e3b0adf1260e4b07 f41b9f44-1aca-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=208529 https://onlinelibrary.wiley.com/doi/10.1155/2019/2815279 0 4f99e163-203a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=209681 Veterinarska fakulteta 0 0 0