20.500.12556/RUL-108751 A combination of electrochemotherapy, gene electrotransfer of plasmid encoding canine IL-12 and cytoreductive surgery in the treatment of canine oral malignant melanoma The aim of this study was to evaluate the safety and efficacy of the combination of electrochemotherapy (ECT) with bleomycin and gene electrotransfer (GET) of plasmid encoding canine interleukin 12 (IL-12) for the treatment of canine oral malignant melanoma (OMM). Our focus was to determine the effect of the treatment on achieving local tumor control and stimulation of an antitumor immune response. Nine dogs with histologically confirmed OMM stage I to III were included in a prospective, non-randomized study. The dogs were treated with a combination of cytoreductive surgery, ECT and IL-12 GET, which was repeated up to five times, depending on the clinical response to the treatment, evaluated according to the follow-up protocol (7, 14 and 28 days after, the last treatment). One month after treatment, the objective response (OR) rate was 67% (6/9). Median survival time (MST) was 6 months and, even though the disease progressed in 8/9 patients at the end of the observation period (2 to 22 months), four animals were euthanized due to tumor-unrelated reasons. In addition, we observed a decline in the percentage of regulatory T cells (Treg) in the peripheral blood in the course of the treatment, which could be attributed to a systemic antitumor response to IL-12 GET. The results of this study suggest that a combination of ECT and IL-12 GET may be beneficial for dogs with OMM, especially when other treatment approaches are not acceptable due to their invasiveness or cost. electrochemotherapy surgery oral interleukin-12 cytoreductive surgery oral malignant melanoma regulatory T cells dogs elektrokemoterapija oralna kirurgija interleukin-12 citoreduktivna kirurgija oralni maligni melanom regulatorne celice T psi true false true Elsevier Angleški jezik Ni določen založnik Znanstveno delo 2019-07-19 09:14:08 2019-07-19 09:14:08 2022-08-20 03:48:15 0000-00-00 00:00:00 2019 0 0 Str. 40-49 Vol. 122 2019 0000-00-00 PostprintKoncna Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2019-11-11 636.7.09:616-006:615 0034-5288 10.1016/j.rvsc.2018.11.001 4730490 26274816 RAZ_Milevoj_Nina_i2019.pdf RAZ_Milevoj_Nina_i2019.pdf 1 E2EABB837BD2D2A85850BBF23D4EA7C0 1fe5f547f82bb01c4d141892062ae3b973f1482b12bc34016792f7c97275ab4b b9a2ca5f-a1b6-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=119880 Veterinarska fakulteta 0 0 0