20.500.12556/RUL-108613 Pregled funkcij proteinskega kompleksa mediator pri rastlinah. Review of the functions of the Mediator complex in plats Uravnavanje prepisa genov je zapleten proces, ki ga vodi množica proteinov in proteinskih kompleksov. Pri evkariontih enega teh predstavlja tudi kompleks mediator, ki sestoji iz okoli 30 proteinov imenovanih MED, ti pa so razporejeni v module. Najprej je bil odkrit v kvasovki Saccharomyces cerevisiae leta 1994, biokemijska identifikacija pri rastlinah pa je bila opravljena šele leta 2007 ob izolaciji kompleksa iz navadnega repnjakovca (Arabidopsis thaliana). Mediator vpliva na prepis tako, da se poveže z RNA polimerazo tipa II in specifičnimi transkripcijskimi faktorji, s tem pa pomaga pri tvorbi prediniciacijskega kompleksa transkripcije. Raziskano je, da mediator s spreminjanjem transkriptoma vpliva na razvoj rastlin, njihov obrambni sistem, na odziv na abiotske stresorje ter na številne druge procese. V delu se osredotočam na vlogo kompleksa pri omenjenih procesih ter na izražanje podenot kompleksa, dotaknem pa se tudi posebnosti rastlinskega mediatorja. Gene transcription is a complex process regulated by many proteins and multiprotein complexes. The Mediator complex is one of the latter and is built up from around 30 MED proteins, which are divided into modules. The complex was discovered in 1994 in the yeast Saccharomyces cerevisiae. The biochemical identification in plants came in 2007, when the complex was isolated from thale cress (Arabidopsis thaliana). Mediator regulates transcription via interactions with RNA polymerase II and specific transcription factors. It has been shown the Mediator complex activity affects plant development, their immunity, response to abiotic stress and many other processes. This is achieved through transcriptome reprogramming. The focus of this work is a review of the aforementioned functions of the complex and the expression of its subunits. I also touch upon the features of the plant Mediator complex. kompleks mediator Arabidopsis thaliana proteinski kompleksi RNA polimeraza II prepis genov Mediator complex Arabidopsis thaliana multiprotein complexes RNA polymerase II gene transcription true false false [T. Kasunič] Slovenski jezik Angleški jezik Diplomsko delo/naloga 2019-07-10 07:45:22 2019-07-10 07:45:24 2022-08-20 03:47:30 0000-00-00 00:00:00 2019 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 601.4:577.21(043.2) 164031 9257849 7320.pdf 7320.pdf 1 C8C6ACA84FCFC59334783DAB19E055F8 dab436159fb174235b2f508fe35305d9426294b1c2c7d3e9d0ed17004630d062 add9de0d-a1b6-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=119593 Biotehniška fakulteta 0 0 0