20.500.12556/RUL-101679 Vpliv svetlobnega režima in temperature na biokemijske pokazatelje fiziološkega stanja v površinskih in jamskih populacijah vodnega oslička Effect of light regimes and temperature on the biomarkers of physiological state in surface and cave waterlouse populations Pred kratkim so raziskovalci merili aktivnost encimov acetilholinesteraza (AChE) in glutation S-transferaza (GST) v vodnih osličkih (Asellus aquaticus, Crustacea, Isopoda), ki so bili nabrani na različnih lokacijah, v različnih letnih časih in opazili veliko variabilnost rezultatov. V nalogi smo želeli ugotoviti, če temperatura vode in svetlobna vplivata na opaženo variabilnost. Nastavili smo tri laboratorijske poskuse, s tremi režimi laboratorijskih pogojev: 11 ºC in tema; 11 ºC in dnevno-nočni svetlobni cikel (16:8 ur sv.:tema) ter 20 ºC in dnevno-nočni svetlobni cikel (16:8 ur sv.:tema). Površinske vodne osličke smo vzorčili dvakrat (23. 2. 2016 in 5. 5. 2016) v močvirnatem gozdu blizu Nacionalnega inštituta za biologijo in z vzorcem izvedli dva laboratorijska poskusa, v katerem smo jih analizirali po 1., 2., in 3. tednu. Jamske vodne osličke smo vzorčili 24. 5. 2017 v Planinski jami in izvedli laboratorijski poskus, v katerem smo analizirali vzorce po 1. in 2. tednu. Ugotovili smo, da se aktivnosti encimov AChE in GST v površinskih in jamskih rakih spremenijo različno, če organizme prenesemo v tri različna okolja. Pri jamskih rakih se na dnevno-nočnem svetlobnem ciklu in 20 ºC poviša aktivnost AChE, GST pa se zmanjša. Pri površinskih rakih se odziv razlikuje glede na starost rakov. Mlajšim se znatno zniža aktivnost GST, vzorec sprememb AChE aktivnost pa ni bil jasno izražen. Pri starejših osebkih se aktivnost GST skoraj ne spremeni, AChE pa ponekod zviša. Nepričakovano nismo opazili razlik med aktivnostjo AChE in GST pri jamskih rakih v primerjavi s površinskimi raki, ki so bili vzorčeni sredi pomladi. Nismo uspeli razložiti, če aktivnost encimov variira zaradi svetlobe in/ali temperature. Recent research had been done by scientists studying enzyme activity of acetylcholinesterase (AChE) and glutation S-transferase (GST) on waterlice (Asellus aquaticus, Crustacea, Isopoda) collected at different locations in different seasons and recorded ample variability in results. We wanted to find out if light and water temperature have an effect on the observed variability of the tested enzyme activities. Three laboratory experiments were set up, using three different regimes of conditions: 11 ºC and darkness; 11 ºC and day-night cycle (16:8h light:darkness); and 20 ºC and day-night cycle (16:8h light:darkness). The surface population was sampled twice (23. 2. 2016 and 5. 5. 2016) in a swampy forest near the National institute of biology, and we ran one experiment after each sampling day. We sampled the subjects after 1, 2 and 3 weeks in the laboratory. We sampled the cave population in Planina cave once (24. 5. 2017) and ran a lab experiment, sampling our subjects after one and two weeks. We found that the activity of AChE and GST changes differently in the three lab environments. In cave animals subjected to 20 ºC and day-night cycle conditions, AChE activity increases and GST activity decreases. In surface animals the response varies due to the age of the test subjects. In older subjects GST doesn't vary much, but AChE is in some cases higher in comparison to the activities in animals collected in the field. Unexpectedly there was no notable difference between enzyme activity of cave animals and older surface animals, sampled in mid-spring. We didn't manage to explain if the enzyme activity in aquatic isopods varies due to light and/or temperature. Asellus aquaticus vodni osliček biomarker laboratorijski poskus jama površinski populacija AChE GST Acetilholinesteraza Glutation-S-transferaza svetlobni režim temperatura Asellus aquaticus waterlouse aquatic sowbug water hoglouse biomarker laboratory experiment cave surface population AChE GST Acetylcholinesterase Glutation-S-transferase light regime temperature true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2018-06-24 07:46:10 2018-06-24 07:46:12 2022-08-14 03:45:43 0000-00-00 00:00:00 2018 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 151372 3129.pdf 3129.pdf 1 2D1DA09E9BC30D2395AE947763D1B675 8dd94f64da2090da733e5ae7ea99b51017f433d5d09ec36edd3f8e2446c5aef7 5ab305a4-a1b5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=111812 Biotehniška fakulteta 0 0 0