20.500.12556/RUL-100805 Vpliv postopka vitrifikacije in zorenja in vitro na izraženost transkripcijskega faktorja FIGLA v humani jajčni celici magistrsko delo, magistrski študij - 2. stopnja Impact of vitrification and in vitro maturation on expression of transcription factor FIGLA in human oocyte M. Sc. Thesis, master study programmes Razlog za neplodnost pri ženskah je včasih lahko tudi slabša kvaliteta jajčnih celic in njihov nepravilni razvoj. Transkripcijski faktor (TF) FIGLA vpliva na tvorbo zone pelucide (ZP) jajčne celice in je vključen v pomembne procese kot sta oogeneza in folikulogeneza. V že opravljenih raziskavah so številni znanstveniki potrdili, da postopki krioprezervacije in zorenja in vitro (IVM) vplivajo na strukturo, delovanje in genetiko jajčne celice, vendar pa v literaturi še ni podatkov o vplivih postopkov zunajtelesne oploditve (IVF) na izražanje TF FIGLA v jajčnih celicah. Ker se neplodnost pogosto zdravi s postopki IVF in ker je za nepravilen razvoj jajčih celic in posledično neplodnost lahko vzrok tudi neizražanje oziroma spremenjeno izražanje TF FIGLA, nas je zanimalo, če in kako postopki IVM, vitrifikacije/devitrifikacije in počasnega zamrzovanja in odmrzovanja vplivajo na izražanje TF FIGLA v skupinah humanih jajčnih celic. Vse celice v raziskavi smo, po določeni metodi, ki se uporablja za IVF, imunocitokemično označili za TF FIGLA in opazovali razlike v vzorcu in jakosti izražanja FIGLA faktorja. Rezultati so pokazali pomembne razlike med jajčnimi celicami, tako v vzorcu izražanja v posameznih celicah kot v jakosti in vzorcu izražanja med skupinami jajčnih celic glede na njihovo zrelost in naštete postopke. Na podlagi tega lahko sklepamo, da imajo postopki IVF vpliv na izražanje tega pomembnega TF, ni pa še znano, ali je ta vpliv posreden ali ne, za kar so potrebne še nadaljnje raziskave. Sometimes female infertility can be a consequence of poor quality of oocytes or their irregular development. Transcription factor (TF) FIGLA has an impact on the formation of zona pellucida (ZP) and is included in important processes like oogenesis and folliculogenesis. In previous studies, many scientists have confirmed that cryopreservation and in vitro maturation (IVM) procedures affect the structure, function and genetics of oocytes, but in literature, there is no information yet on the impact of in vitro fertilisation (IVF) procedures on the expression of TF FIGLA in human oocytes. Treatment of infertility with IVF procedures and the fact that irregular oocyte development and infertility can depend on non-expression or changed expression of TF FIGLA, made us wonder if and how the procedures of IVM, vitrification/devitrification and slow freezing and warming affect the expression of TF FIGLA in different groups of human oocytes. All cells included in our research were, according to a specific procedure used for IVF, immunocytochemically marked as TF FIGLA and then the differences in pattern and strength of TF FIGLA expression were observed. The results showed some significant differences between oocytes: in their expression pattern in a singular cell and also in the expression of strength and pattern between different groups of cells, considering their maturity and previously mentioned procedures. Based on that, we can assume that IVF procedures affect the TF FIGLA expression, yet it is still unknown if these effects are indirect or not, for which further research is needed. neplodnost TF FIGLA humana jajčna celica vitrifikacija IVM vplivi infertility TF FIGLA human oocyte vitrification IVM effects true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2018-04-16 15:40:31 2018-04-16 15:40:31 2022-08-14 03:42:00 0000-00-00 00:00:00 2018 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 2019-04-16 151148 4668239 2416.pdf 2416.pdf 1 D02E6B59069B8BE94A3C1C57C95B6302 abb0556e43bae38ea994fd970eea9dd289c20d74ed60cd1bbfde1ef1ab69a455 1d6ba27a-a1b5-11eb-a523-00155dcfd717 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=110607 Biotehniška fakulteta 0 0 0