20.500.12556/RUL-100079 Radialna porazdelitev kreozotnega olja v impregnirani bukovini in njen vpliv na fungicidno učinkovitost Radial distribution of creosote in impregnated beech wood and its influence on fungicidal properties Kreozotno olje se že 200 let uporablja predvsem za zaščito lesa v stiku z zemljo, ki je še posebej izpostavljen razkroju gliv. Najpogosteje se uporablja za impregnacijo železniških pragov, ki so v Sloveniji izdelani večinoma iz bukovine. V okviru naše naloge smo želeli raziskati fungicidno učinkovitost kreozotnega olja in osvetliti vpliv anatomskih značilnosti na odpornost z njim impregnirane bukovine, namenjene izdelavi železniških pragov. Vzorcem impregniranega lesa smo določili maso v suhem stanju. Z ekstrakcijo po Soxhletu, pri čemer smo uporabili mešanico n-heksana in 96 % etanola, smo polovici vzorcev določili navzem s kreozotnim oljem, drugo polovico vzorcev smo izpostavili glivam bele trohnobe (Trametes versicolor, Hypoxylon fragiforme in Pleurotus ostreatus) v skladu z modificiranim standardom SIST EN 113. Pred tem smo vzorce starali po standardu SIST EN 84. Po 16-tedenski izpostavitvi smo vzorcem določili izgubo mase in izračunali povezanost med navzemom kreozotnega olja in fungicidno učinkovitostjo. Navzem s kreozotnim oljem ni bil višji na robovih pragov in nižji na sredini, kot smo predvidevali v prvi hipotezi, v skladu z drugo hipotezo pa je bil navzem manjši na delih vzorcev, ki so vsebovali rdeče srce bukovine. Potrdili smo tretjo hipotezo, saj so bili deli z nezadostnim navzemom kreozotnega olja manj zaščiteni pred delovanjem lesnih gliv. Ugotovitve lahko upoštevamo pri nadaljnjih impregnacijskih postopkih. Creosote oil is used as a wood preservative last 200 year. Especially when it’s in touch with earth where exposure to rot is high, like railroad sleepers, that in Slovenia mostly made out of beach wood. In our study, we wanted to enlighten the antifungal properties of creosote oil and the influence of anatomic features of beach wood that was impregnated with creosote oil, intended for railroad sleepers. We measured the weight of the samples of impregnated wood in absolute dray environment. With extraction after Soxhlet, we used a mixture of n-hexane and 96 % ethanol, we determined in half of the samples the creosote oil uptake, the other half we exposed to white-rot fungi (Trametes versicolor, Hypoxylon fragiforme in Pleurotus ostreatus) in line with standard SIST EN 113. Before that we artificially aged the samples by standard SIST EN 84. After 16 weeks of exposure we determined the weight loss and a link between creosote oil uptake and antifungal properties. Creosote oil uptake was not higher on the edges and lower in the middle of the railroad sleeper like we anticipated in our first hypothesis. In according with our second hypothesis the creosote oil uptake was lower in the regions were beech red heartwood was present. We confirmed our third hypothesis, that the area with less creosote oil uptake was more susceptible to rot. The findings can be used in future wood impregnation procedures. kreozotno olje fungicidne lastnosti železniški prag creosote oil fungicidal properties railway tie true false false [M. Pajk] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2018-03-04 07:15:06 2018-03-04 07:15:12 2022-08-24 17:15:44 0000-00-00 00:00:00 2018 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*841.11 150992 2882441 2338.pdf 2338.pdf 1 DDC60F613B0408E1F490D5A1359E60B0 3faf00b1e55a589b8351d746cf232e224765acf5bf8ec6f066eaca146b035d94 ab58aac5-a1b4-11eb-a523-00155dcfd717 20.500.12556/rul/c8daa46f-9666-41d0-bd5a-3c20f50c74e7 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=109738 Biotehniška fakulteta 0 0 0