Bioinformatic evidence and characterization of novel putative large conjugative transposons residing in genomes of genera Bacteroides and PrevotellaGorenc, Katja (Avtor) Accetto, Tomaž (Avtor) Avguštin, Gorazd (Avtor) mikrobiologijaBacteroidesPrevotellamolekularna genetikagenombioinformatikaBioinformatic evidence of the presence of a large conjugative transposon in ruminal bacterium Prevotella bryantii B[sub]14[sup]T is presented. The described transposon appears to be related to another large conjugative transposon CTnBST, described in Bacteroides uniformis WH207 and to the conjugative transposon CTn3-Bf, which was observed in the genome of Bacteroides fragilis strain YCH46. All three transposons share tra gene regions with high amino acid identity and clearly conserved gene order. Additionally, a second conserved region consisting of hypothetical genes was discovered in all three transposons and named the GG region. This region served as a specific sequence signature and made possible the discovery of several other apparently related hypothetical conjugative transposons in bacteria from the genus Bacteroides. A cluster of genes involved in sugar utilization and metabolism was discovered within the hypothetical CTnB[sub]14, to a certain extent resembling the polysaccharide utilization loci which were described recently in some Bacteroides strains. This is the first firm report on the presence of a large mobile genetic element in any strain from the genus Prevotella.20122015-07-10 12:30:30Delo ni kategorizirano36560UDK: 579:575ISSN pri članku: 0015-5632DOI: 10.1007/s12223-012-0126-5COBISS_ID: 3042440OceCobissID: 25470976sl