Chemiluminescence of non-differentiated THP-1 promocytesTompa, Gorazd (Avtor) Laine, Aire (Avtor) Pihlanto, Anne (Avtor) Korhonen, Hannu (Avtor) Rogelj, Irena (Avtor) Marnila, Pertti (Avtor) mlekobeljakovinepeptidianalizna kemijakemiluminiscencaHuman leukemic THP-1 promonocytes are widely used as a model for peripheral blood monocytes. However, superoxide production during respiratory burst (RB) of non-differentiated THP-1 (nd-THP-1) cells is very low. Here we present a rapid and low-cost method for measuring the chemiluminescence (CL) of opsonized zymosan (OZ) induced RB which allows detection of Escherichia coli lipopolysaccharide (LPS) induced priming of nd-THP-1 cells on the basis of CL reaction kinetics. Maximum CL intensity obtained was 2.20+- 0.25 and 1.30+- 0.11 relative light units, while CL peak time was achieved at 18.1+- 2.6 and 28.7+- 1.3 min in primed and non-primed cells, respectively. The priming of nd-THP-1 cells with LPS evoked tzpical TNF-[alfa] and IL-6 production. We tested the effects of bovine lactoferrin and protein fractions from Lactobacillus helveticus BGRA43 fermented milk for potential anti-inflammatory effects on LPS primed nd-THP-1 cells. Four fractions were found to inhibit the OZ-induced CL in a dose-dependent manner (IC50 3-30 g/mL), while lactoferrin inhibited CL to a lesser extent (IC50 270? g/mL). These results suggest that measuring CL response of nd-THP-1 cells can serve as a method for screening anti-inflammatory compounds which could be used in reducing the risk of phagocyte-mediated inflammatory diseases.20112015-07-10 12:30:08Delo ni kategorizirano36531UDK: 637.1:543ISSN pri članku: 1522-7235DOI: 10.1002/bio.1220COBISS_ID: 2757768OceCobissID: 436108sl