Season-long lateral-shoot removal alters fruit volatiles and glucose content in primocane-fruiting raspberry (Rubus idaeus L. cv. ‘Enrosadira’)Čučkin, Hana (Avtor) Jakopič, Jerneja (Avtor) Grohar, Mariana Cecilia (Avtor) pruninglateral shootscanopy architecturequalityPruning is an essential horticultural practice used to shape raspberry plant architecture, with consequent effects on the balance between vegetative growth and fruit production. In this study, we investigate for the first time the effects of removing lateral shoots in the primocane-fruiting raspberry cultivar ‘Enrosadira’. In branched plants, lateral shoots were retained, whereas in unbranched plants laterals were removed throughout the season. We evaluated yield, vegetative plant parameters (height, leaf surface area, number of leaflets, net photosynthesis), and targeted primary-metabolite and volatile profiling (sugars, organic acids, ascorbic acid, phenolic compounds and volatile organic compounds), using HPLC and GC-MS. Glucose content was up to 45% higher in fruits from branched plants right before the seasonal peak. Statistically higher values were observed in the fruits from the unbranched treatment for total aldehydes compared to the branched treatment. Furthermore, several individual volatiles were significantly higher in the unbranched treatment, including five aldehydes (hexanal, hex-3-enal, hex-(2E)-enal, decanal, and dodecanal), three monoterpenes (α-pinene, α-phellandrene, and β-phellandrene), octanoic acid, and α-ionol, a key contributor to raspberry aroma. Under the conditions tested, lateral-shoot removal did not affect yield or the measured vegetative traits but altered selected key quality fruit metabolites of primocane-fruiting raspberry cultivar ‘Enrosadira’.20262026-10-14 13:10:30Raziskovalni podatki189748slThe present study was conducted in the experimental field of the Biotechnical Faculty of the University of Ljubljana, Slovenia (latitude: 46°02’ N; longitude: 14°28’ E; altitude: 295 m a.s.l.).The present study on the seasonal removal of lateral shoots on primocane-fruiting raspberry (Rubus idaeus L.) was conducted from June to November 2025.