Smoking and diabetes attenuate number of CD34$^{+}$ haematopoietic stem cells in peripheral blood of patients with advanced peripheral artery diseaseŠernek, Barbara (Avtor) Kamnikar, Rok (Avtor) Šebeštjen, Miran (Avtor) Boc, Anja (Avtor) Boc, Vinko (Avtor) CD34$^{+}$ cellsperipheral artery diseasesmokingdiabetes type IIintermittent claudicationPeripheral artery disease (PAD) is a globally prevalent problem with limited treatment options, leaving up to a fifth of patients remediless. The emergence of new studies on cell therapy in recent years offers a new promising option for their treatment. Our aim was to explore how the number of CD34$^{+}$ hematopoietic cells in the peripheral blood of PAD patients is associated with patients’ functional as well as atherogenic factors. We selected 30 patients with advanced PAD, recorded their performance in a walking test in standard conditions and sampled their blood for further analysis with an emphasis on CD34$^{+}$ cell selection and counting. No correlation of the CD34$^{+}$ cell number was confirmed with any of the observed laboratory parameters. There was an association between the claudication distance and the number of CD34$^{+}$ cells (r = −0.403, p = 0.046). The number of CD34$^{+}$ cells differed between patients with and without type II diabetes (p = 0.071) and between active smokers, past smokers, and non-smokers (p = 0.035; p = 0.068, p = 0.051, respectively), with both smoking and presence of diabetes type II having a negative effect on the number of CD34$^{+}$ cells. Our study demonstrated a dependence of the CD34$^{+}$ cell number on the patient’s characteristics.20232026-09-13 12:12:13Članek v reviji187756UDK: 616.1ISSN pri članku: 1422-0067DOI: 10.3390/ijms242015346COBISS_ID: 177470723sl