Multiscale imaging of Pleurotus ostreatus fruiting bodies – from 3D anatomy to the ultrastructureMrak, Polona (Avtor) Conti, Jacopo (Avtor) Bogataj, Urban (Avtor) Skočaj, Matej (Avtor) Kostanjšek, Rok (Avtor) Žnidaršič, Nada (Avtor) micro-CTmicroscopyfungihyphaecell wallseptal poreOyster mushroom, Pleurotus ostreatus, is economically important and widely used as a model organism, yet a comprehensive characterisation of its fruiting body structure across multiple levels of biological organisation is lacking. We applied a multiscale imaging, combining micro-computed tomography (micro-CT), light microscopy of paraffin, cryostat and semithin sections, and transmission electron microscopy to investigate the structure of P. ostreatus young fruiting bodies, and we provide a direct comparison of different sample preparation procedures. Micro-CT enabled 3D anatomy visualisation, revealing distinct tissue regions: a loose trama in the stipe and pileus medulla, a dense peripheral layer of pileus and gills, and a thin, loose layer covering pileus and gills. Histological analyses provided detailed information on tissue organisation, complementing the micro-CT images, including hyphal arrangement, gill architecture, hymenial layer, and basidia. Cryosections preserved overall tissue morphology and proved suitable for future molecular localisation studies. Transmission electron microscopy of Agar 100 and LR White embedded samples resolved the ultrastructure of hyphae and basidia, revealing nuclei, mitochondria, endoplasmic reticulum, vacuoles, cell walls, and dolipore septa with perforated pore cap. The study demonstrates the value of integrating imaging techniques to characterise the mushroom structure across scales and provides a toolbox for future investigations of fruiting bodies.20262026-08-06 08:46:50Članek v reviji185464UDK: 543.456ISSN pri članku: 1854-3073DOI: 10.14720/abs.69.3.28176COBISS_ID: 286891523sl