Design, synthesis and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitorsPurić, Edvin (Avtor) Marinovich, Marina (Avtor) Kojek, Zala (Avtor) Kahl-Knutson, Barbro (Avtor) Leffler, Hakon (Avtor) Nilsson, Ulf (Avtor) Anderluh, Marko (Avtor) Mravljak, Janez (Avtor) fluorescence polarisation assaycarbohydrateslectinsgalectin-8In the present work, we describe the design, synthesis and evaluation of a galectin-8-binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its photophysical properties and its binding affinity for galectin-8N was determined by using FP. We evaluated the probe in a competitive FP assay with three known galectin-8N inhibitors and demonstrated its suitability for high-throughput screening.20252025-06-30 09:32:06Članek v reviji169974UDK: 615.4:54ISSN pri članku: 1846-9558DOI: 10.2478/acph-2025-0010COBISS_ID: 233259779sl