Phenolics and cyanogenic glycosides in the ethanolic extract of black cherry Prunus serotina Ehrh. fruitsBrozdowski, Jakub (Avtor) Veberič, Robert (Avtor) Mikulič Petkovšek, Maja (Avtor) bioactive compoundsphenolicscyanogenic glycosidesextractioninvasive plant speciesinvasive plantsforestsBlack cherryPrunus serotineEuropeBlack cherry is an invasive alien plant species that has spread throughout Europe. Various meth− ods are used to control its population including the removal of Prunus serotina biomass from forests. Black cherry trees bear fruit at a remarkably young age, usually between 3 and 5 years old, and produce an abundance of small black fruits that resemble cherries. Harvesting these fruits can help reduce the growth of the population. Harvested fruits can also be processed to extract valuable secondary plant substances, especially phenolics. This study compares the success of one and two week ethanolic extraction methods of black cherry fruit from two growing locations. To determine the content of bioactive compounds in fruit, the biomass fruit was extracted in ethanol solution. Following extraction, the content of bioactive compounds was determined using high−performance liquid chromatography (HPLC) equipped with two detectors. A mass spectrometry detector (MS$^n$) was used for qualitative analysis, while a diode array detector (DAD) was employed for quantitative determination. In total between 8614 and 10780 µg/ml of phenolics were extracted. The results show that the content of some groups of phenolics was influenced by time, namely flavonols and anthocyanins, while the content of flavanones and derivatives of hydroxycinnamic acid was influenced by the location of the harvest. When the fruits were soaked in alcohol for two weeks, the content of cyanogenic glycosides was not influ− enced by time or location. Cyanogenic glycosides content after two weeks of soaking in alcohol ranged from 31.6 to 32.9 µg/ml.20252025-06-16 11:26:56Članek v reviji169916UDK: 543.2:582.639.41ISSN pri članku: 0039-7660DOI: 10.26202/sylwan.2024067COBISS_ID: 239206915sl