Performance evaluation of a novel MBT LipidART module (Bruker Daltonics) for detection of colistin resistance in Escherichia coli and Klebsiella pneumoniaeGerm, Julija (Avtor) Pirš, Mateja (Avtor) performance evaluationMBT LipidART moduledetection of colistin resistanceEscherichia coliKlebsiella pneumoniaeWe evaluated the MBT LipidART module on a MALDI Biotyper® Sirius System (Bruker Daltonics) for the rapid detection of colistin resistance in Escherichia coli (EC) and Klebsiella pneumoniae (KPN) by analysing lipid A profiles in negative ion mode. Categorical agreement was achieved for 98.3% EC (N = 58) and 85.0% KPN (N = 40). Challenges included calibration difficulties, limited availability of compatible equipment and issues with mucoid and adherent KPN isolates that yielded invalid results. While MBT LipidART module shows promise as a rapid tool for detection of colistin resistance, its performance was notably better for EC compared to KPN.20252025-05-28 11:42:45Članek v reviji169431UDK: 616-097:616.1ISSN pri članku: 0934-9723DOI: 10.1007/s10096-025-05099-4COBISS_ID: 230357763sl