Sustainable furfural valorizationBožinović, Marko (Avtor) Jereb, Marjan (Avtor) Šketa, Borut (Avtor) Gaber, Aljaž (Avtor) Seručnik, Mojca (Avtor) Košmrlj, Janez (Avtor) Žnidaršič Plazl, Polona (Avtor) furfuralfurfurylaminetransaminasebiomass valorizationgreen chemistryThe growing global demand for furfurylamine (FA) underscores the need for environmentally friendly production methods. To address this, we explored a biocatalytic approach using transaminase (TA) for furfural (FUR) amination. Our main goal was to find an effective enzyme and cost-efficient amine donor for FA production. Screening various enzymes and amine donors revealed that FA synthesis occurred only with a tetrameric $N$-His$_6$-ATA-wt enzyme and ($S$)-(−)-α-methylbenzylamine (MBA) as the amine donor at equimolar concentrations. This method yielded 96% FA in 30 minutes, the highest reported efficiency. The proposed reaction operates at 30°C with minimal energy and material consumption, offering a promising sustainable route for FA synthesis. 20242024-07-22 11:46:16Neznano159748sl