Bacterial DNA recognition by SERS active plasma-coupled nanogoldShvalya, Vasyl (Avtor) Skubic, Cene (Avtor) Nadižar, Nejc (Avtor) Rozman, Damjana (Avtor) Cvelbar, Uroš (Avtor) DNA genomic rationplasma electrochemical reductioncoupled plasmonic nanogoldDNA Raman fingerprintsgeneticsgenomicsgoldplasmaRaman spectroscopyIt is shown that surface-enhanced Raman spectroscopy (SERS) can identify bacteria based on their genomic DNA composition, acting as a “sample-distinguishing marker”. Successful spectral differentiation of bacterial species was accomplished with nanogold aggregates synthesized through single-step plasma reduction of the ionic gold-containing vapored precursor. A high enhancement factor (EF = 10$^7$) in truncated coupled plasmonic particulates allowed SERS-probing at nanogram sample quantities. Simulations confirmed the occurrence of the strongest electric field confinement within nanometric gaps between gold dimers/chains from where the molecular fingerprints of bacterial DNA fragments gained photon scattering enhancement. The most prominent Raman modes linked to fundamental base-pair molecular vibrations were deconvoluted and used to proceed with nitrogenous base content estimation. The genomic composition (percentage of guanine-cytosine and adenine-thymine) was successfully validated by third-generation sequencing using nanopore technology, further proving that the SERS technique can be employed to swiftly specify bioentities by the discriminative principal-component statistical approach.20222023-02-14 10:11:00Članek v reviji144312UDK: 533ISSN pri članku: 1530-6992DOI: 10.1021/acs.nanolett.2c02835COBISS_ID: 127669763sl