Experimental exposure to bisphenol A has minimal effects on bone tissue in growing ramsBrankovič, Jana (Avtor) Leskovec, Jakob (Avtor) Šturm, Sabina (Avtor) Cerkvenik, Vesna (Avtor) Šterpin, Saša (Avtor) Osredkar, Joško (Avtor) Pogorevc, Estera (Avtor) Antolinc, David (Avtor) Vrecl, Milka (Avtor) bisphenol Aramsserum bone biomarkersbone geometrytrabecular bonecortical bonebone biomechanicsmineral contentBisphenol A (BPA) is a well-known synthetic compound that belongs to the group of endocrine-disrupting chemicals. Although bone tissue is a target for these compounds, studies on BPA-related effects on bone morphology in farm animals are limited. In this preliminary study, we investigated the effects of short-term dietary BPA exposure on femoral morphology, metabolism, mineral content, and biomechanical behavior in rams aged 9–12 months. Fourteen rams of the Istrian Pramenka breed were randomly divided into a BPA group and a control group (seven rams/group) and exposed to 25 μg BPA/kg bw for 64 days in feed. Blood was collected for determination of bone turnover markers (procollagen N-terminal propeptide, C-terminal telopeptide), and femurs were assessed via computed tomography, histomorphometry, three-point bending test, and mineral analysis. BPA had no significant effects on most of the parameters studied. Only mineral analysis showed decreased manganese (50%; p ≤ 0.05) and increased copper content (25%; p ≤ 0.05) in the femurs of BPA-exposed rams. These results suggest that a 2-month, low-dose exposure to BPA in growing rams did not affect the histomorphology, metabolism, and biomechanical behavior of femurs; however, it affected the composition of microelements, which could affect the histometric and biophysical properties of bone in the long term.20222022-08-26 07:38:11Neznano138935UDK: 636.09:615.9ISSN pri članku: 2076-2615DOI: 10.3390/ani12172179COBISS_ID: 119202307sl