2-acyl-1-aryl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivativesPetek, Nejc (Avtor) Erjavec, Bibi (Avtor) Slapšak, Dejan (Avtor) Gaber, Aljaž (Avtor) Grošelj, Uroš (Avtor) Požgan, Franc (Avtor) Ričko, Sebastijan (Avtor) Štefane, Bogdan (Avtor) Klemenčič, Marina (Avtor) Svete, Jurij (Avtor) 67-dihydro-1H5H-pyrazolo[12-a]pyrazolesbenzotriazolidesfluorescent imagingbioconjugationsolvatochromismA series of thirteen 2-acyl-6,7-dihydro-1H,5H-pyrazolo[1,2-a]pyrazole derivatives were synthesized by using a copper-catalyzed azomethine-imine-alkyne cycloaddition as a key step. These bimane-related fluorescent compounds with λ$_{ex}$ = 338–400 nm and λ$_{em}$ = 522–644 nm exhibit interesting and promising optical properties, including a remarkably large Stokes shift (150–268 nm), positive solvatochromism, and strong dependence of emission intensity on pH in a range of pH 2–10. The benzotriazolide- and N-succinimidyl ester-functionalized compounds were successfully used as fluorescent probes for protein labelling.20222022-06-23 14:30:49Članek v reviji137625UDK: 547.77ISSN pri članku: 0143-7208DOI: 10.1016/j.dyepig.2022.110224COBISS_ID: 100346115sl