Hydrogel-based enzyme and cofactor co-immobilization for efficient continuous transamination in a microbioreactorMenegatti, Tadej (Avtor) Žnidaršič Plazl, Polona (Avtor) cofactor co-immobilizationhydrogelmicroreactoramine transaminasecontinuous processA microbioreactor was developed in which selected amine transaminase was immobilized together with the cofactor pyridoxal phosphate (PLP) to allow efficient continuous transamination. The enzyme and cofactor were retained in a porous copolymeric hydrogel matrix formed in a two-plate microreactor with an immobilization efficiency of over 97%. After 10 days of continuous operation, 92% of the initial productivity was retained and no leaching of PLP or enzyme from the hydrogel was observed. The microbioreactor with co-immobilized cofactor showed similar performance with and without the addition of exogenous PLP, suggesting that the addition of PLP is not required during the process. The space-time yield of the microbioreactor was 19.91 g L−1 h−1, while the highest achieved biocatalyst productivity was 5.4 mg mgenzyme−1 h−1. The immobilized enzyme also showed better stability over a wider pH and temperature range than the free enzyme. Considering the time and cost efficiency of the immobilization process and the possibility of capacity expansion, such a system is of great potential for industrial application.20212022-04-15 08:32:00Članek v reviji136179UDK: 66.098ISSN pri članku: 2296-4185DOI: 10.3389/fbioe.2021.752064COBISS_ID: 83665411sl