Characterisation of lactoferrin isolated from acid whey using pilot-scale monolithic ion-exchange chromatographyBogovič Matijašić, Bojana (Avtor) Oberčkal, Jernej (Avtor) Mohar Lorbeg, Petra (Avtor) Paveljšek, Diana (Avtor) Skale, Nina (Avtor) Kolenc, Borut (Avtor) Gruden, Špela (Avtor) Poklar Ulrih, Nataša (Avtor) Kete, Marko (Avtor) Zupančič Justin, Maja (Avtor) lactoferrinacid wheymonolithic ion-exchange chromatographyE. coli O157:H7The aim of this study was to characterize the properties of lactoferrin (LF) obtained in a process developed for its isolation from acid whey derived from the production of fresh curd cheese, using a unique technology of ion-exchange chromatography on CIM$^®$ monolithic columns. The freeze-dried lactoferrin samples produced on the pilot plant (capacity 1 m$^3$) were examined for the purity, iron-binding capacity, antibacterial activity, and pH- and temperature-stability. Apo-LF inhibited several tested strains (enterobacteria, Staphylococcus, Streptococcus salivarius) except clostridia, lactic acid bacteria, and bifidobacteria. Sample of LF intentionally saturated with Fe$^{3+}$ lost its antibacterial activity, indicating the involvement of mechanisms based on depriving bacteria of an iron source. All samples, regardless of the iron-saturation level, exhibited stability in pH range 4.0 to 11.0. LF with higher iron content (A-value = 41.9%) showed better thermal stability. Heat treatment up to 72 °C/3 s did not reduce antimicrobial activity against E. coli O157: H7 tox-. Higher purity (above 91%), higher iron-binding capacity and higher inhibitory activity against E. coli O157: H7 tox- compared to some similar products from the market was observed. These results demonstrate a high potential of monolithic ion-exchange chromatography for industrial processing of acid whey as a source of LF that can be used in new products with high-added value. The upscaling of the process is ongoing on a demonstration plant (10–30 m$^3$/day capacity).20202021-11-03 09:00:25Članek v reviji132775UDK: 579.67:637.1:543.544.14ISSN pri članku: 2227-9717DOI: 10.3390/pr8070804COBISS_ID: 23698947sl